Thermo Fisher Scientific SAE2 Polyclonal Antibody
Thermo Fisher Scientific SAE2 Polyclonal Antibody는 Human 및 Mouse 시료에서 SAE2 단백질 검출에 적합한 Rabbit IgG 폴리클로날 항체입니다. WB, IHC, IP 등 다양한 응용에 사용 가능하며, 항원 친화 크로마토그래피로 정제된 고품질 제품입니다. 연구용으로만 사용됩니다.
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Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:2,000–1:10,000 |
| Immunohistochemistry (IHC) | 1:100–1:500 |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:100–1:500 |
| Immunoprecipitation (IP) | 2–5 µg/mg lysate |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Human, Mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Region between residue 525 and 575 of Human SUMO1 Activating Enzyme Subunit 2 (UBA2, UniProt ID: Q9UBT2-1) |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 0.20 mg/mL |
| Purification | Antigen affinity chromatography |
| Storage Buffer | TBS, pH 7.0–8.0, with 0.1% BSA |
| Contains | 0.09% sodium azide |
| Storage Conditions | 4°C |
| Shipping Conditions | Wet ice |
Product Specific Information
- Recommended shelf life: 1 year from date of receipt
- Application Note: For IHC, epitope retrieval with citrate buffer (pH 6.0) is recommended for FFPE tissue sections.
Target Information
UBA2 is involved in the covalent attachment of one protein to another, a major posttranslational modification. Ubiquitin is the most well-known modifier, and its activation and transfer have been extensively studied. SUMO (Sentrin) is a ubiquitin-like protein implicated in stabilization and localization of target proteins. The conjugation of SUMO-1, SUMO-2, and SUMO-3 onto target proteins requires the coordinated action of the E1-activating enzyme SAE1/SAE2, the E2-conjugating enzyme Ubc9, and an E3-like SUMO ligase.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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