Thermo Fisher Scientific CD45R (B220) Monoclonal Antibody (RA3-6B2), Functional Grade, eBioscience
Mouse CD45R(B220) 단백질을 인식하는 RA3-6B2 클론의 Rat IgG2a 단일클론 항체로, Flow Cytometry 및 기능적 분석에 적합. 1 mg/mL 액상 형태, 4°C 보관. 높은 순도와 낮은 엔도톡신 수준으로 B세포 연구에 신뢰성 제공.
- 카탈로그번호
- 16-0452-xxx (6개 옵션)
- 판매단위
- pk
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6개 옵션 · 카탈로그 번호를 클릭하면 복사됩니다Thermo Fisher Scientific · Thermo Fisher Scientific CD45R (B220) Monoclonal Antibody (RA3-6B2), Functional Grade, eBioscience
Thermo Fisher Scientific CD45R (B220) Monoclonal Antibody (RA3-6B2), Functional Grade, eBioscience
Applications and Tested Dilutions
| Application | Tested Dilution | Publications |
|---|---|---|
| Immunohistochemistry (IHC) | - | 36 |
| Immunohistochemistry (Paraffin) (IHC (P)) | - | 3 |
| Immunohistochemistry (Frozen) (IHC (F)) | - | 2 |
| Immunocytochemistry (ICC/IF) | - | 6 |
| Flow Cytometry (Flow) | 0.5 µg/test | 79 |
| Functional Assay | Assay-Dependent | 1 |
| Miscellaneous PubMed | - | 1 |
Product Specifications
| Specification | Details |
|---|---|
| Published Species | Fish, Human, Mouse |
| Host / Isotype | Rat / IgG2a, kappa |
| Recommended Isotype Control | Rat IgG2a kappa Isotype Control (eBR2a), Functional Grade |
| Class | Monoclonal |
| Type | Antibody |
| Clone | RA3-6B2 |
| Conjugate | Functional Grade |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Storage Conditions | 4°C |
| Shipping Conditions | Wet Ice |
| RRID | AB_2865732 |
Available Conjugate Formats
- Unconjugated
- Alexa Fluor 488, 532, 561, 660, 700
- APC, APC-eFluor 780, Biotin
- Brilliant UV series (395–805)
- Brilliant Violet series (421–786)
- eFluor series (450–660)
- FITC, PE, PE-Cyanine5, PE-Cyanine7, PerCP-eFluor 710
- Super Bright series (436–780)
- NovaFluor series (Blue, Red, Violet, Yellow, UV variants)
- Custom conjugation available upon request
Product Specific Information
Description:
The RA3-6B2 monoclonal antibody reacts with the exon A-restricted isoform of mouse CD45 (220 kDa surface molecule). CD45R/B220 is primarily expressed by B cell lineage from early Pro-B to mature B cells and can also be found on activated T cells, LAK cells, NK progenitors, and certain mutant T cells (lpr/lpr).
Applications Reported:
- Flow cytometric analysis
- Functional studies of B cells
Applications Tested:
Validated for flow cytometric analysis of mouse splenocytes at ≤ 0.5 µg/test (100 µL final volume). Recommended titration for optimal assay performance.
Storage and Handling:
- Use in sterile environment
- 0.2 µm post-manufacturing filtration
- Purity >90% (SDS-PAGE)
- Endotoxin <0.001 ng/µg (LAL assay)
- Aggregation <10% (HPLC)
Target Information
CD45R (PTPRC) is a protein tyrosine phosphatase involved in cell signaling, growth, differentiation, and immune regulation. It is expressed in hematopoietic cells and regulates T- and B-cell receptor signaling. CD45 isoforms arise via alternative splicing, influencing membrane localization and expression patterns. Dysfunction of CD45 is associated with immunodeficiency and hepatitis C.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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