Thermo Fisher Scientific AR Monoclonal Antibody (OTI2E11), TrueMAB

Applications

Western Blot (WB)

• Tested Dilution: 1:2,000

Immunohistochemistry (Paraffin) (IHC (P))

• Tested Dilution: 1:1,000

Product Specifications

Product Specific Information

For reconstitution, add 100 µL distilled water to achieve a final antibody concentration of approximately 1 mg/mL.
For conjugation experiments, perform an additional desalting step using Zeba Spin Desalting Columns (7K MWCO, 0.5 mL, Product #89882).

Target Information

The androgen receptor (AR) is a member of the steroid-hormone receptor superfamily of nuclear receptors.
It has three major functional domains: the N-terminal domain, DNA-binding domain, and androgen-binding domain.
AR acts as a ligand-activated transcription factor binding testosterone (T) and dihydrotestosterone (DHT).
Upon ligand binding, AR translocates into the nucleus, dimerizes, and activates transcription of androgen-responsive genes.

The AR signaling pathway is crucial for development and function of male reproductive organs (prostate, epididymis) and also affects nonreproductive tissues (muscle, hair follicles, brain).
AR is a phosphoprotein regulating MAP kinase signaling, and its phosphorylation state is linked to MEK1/2 pathway activity.
Abnormal AR signaling is associated with diseases such as prostate cancer, Kennedy’s disease, and male infertility.
Mutations in AR can cause complete androgen insensitivity (CAIS).

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.