Thermo Fisher Scientific Sulfotyrosine Monoclonal Antibody (7C5), PerCP
Sulfotyrosine 단백질의 황산화 타이로신 잔기를 특이적으로 인식하는 단클론 항체. PerCP로 결합되어 형광 검출 가능. Western blot, IHC, ICC/IF, ELISA에 사용 가능. 마우스 IgG1 형식으로 높은 특이성과 재현성을 제공.
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Applications and Tested Dilutions
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:1,000 |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:25 |
| Immunocytochemistry (ICC/IF) | 1:50 |
| ELISA | 1:1,000 |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Chemical |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Clone | 7C5 |
| Immunogen | Synthetic Sulfotyrosine conjugated to Keyhole Limpet Hemocyanin (KLH) |
| Conjugate | PerCP |
| Excitation / Emission Max | 482 / 675 nm |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Protein G |
| Storage Buffer | 95.64 mM phosphate / 2.48 mM MES, pH 7.4, with 0.5 M EDTA |
| Contains | No preservative |
| Storage Conditions | 4°C |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2932289 |
Additional Formats
Product Specific Information
A 1:1,000 dilution of MA5-45835 was sufficient for detection of Sulfotyrosine in 0.5 µg of Sulfotyrosine conjugated to BSA by ECL immunoblot analysis using Goat Anti-Mouse IgG:HRP as the secondary antibody.
Specific for sulfated tyrosine residues on various proteins. Does not detect free Sulfotyrosine. Does not cross-react with phosphotyrosine.
Target Information
Sulfotyrosine is involved in protein-protein interactions. Sulfotyrosines are present in all multicellular eukaryotes, but unicellular eukaryotes such as yeast and prokaryotes are not capable of tyrosine sulfation.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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