Thermo Fisher Scientific Sulfotyrosine Monoclonal Antibody (7C5), APC
Sulfotyrosine 특이적 단클론 항체로 다양한 단백질 내 황산화 티로신 잔기 검출에 사용. Western blot, IHC, ICC, ELISA에 적합. APC 형광 표지로 651/660 nm 파장 사용. 단백질 G 정제, 보존제 무첨가, 4°C 보관.
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Applications and Tested Dilutions
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:1,000 |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:25 |
| Immunocytochemistry (ICC/IF) | 1:50 |
| ELISA | 1:1,000 |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Chemical |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Clone | 7C5 |
| Immunogen | Synthetic Sulfotyrosine conjugated to Keyhole Limpet Hemocyanin (KLH) |
| Conjugate | APC |
| Excitation / Emission Max | 651 / 660 nm |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Protein G |
| Storage Buffer | 95.64 mM phosphate / 2.48 mM MES, pH 7.4, with 0.5 M EDTA |
| Contains | No preservative |
| Storage Conditions | 4°C |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2932287 |
Available Formats
- Unconjugated (MA5-27551)
- FITC (MA5-45834)
- PE (MA5-45836)
- PerCP (MA5-45835)
- Custom conjugation available upon request
Product Specific Information
A 1:1,000 dilution of MA5-45833 was sufficient for detection of Sulfotyrosine in 0.5 µg of Sulfotyrosine conjugated to BSA by ECL immunoblot analysis using Goat Anti-Mouse IgG:HRP as the secondary antibody.
Specific for sulfated tyrosine residues on various proteins. Does not detect free Sulfotyrosine. Does not cross-react with phosphotyrosine.
Target Information
Sulfotyrosine is involved in protein-protein interactions. Sulfotyrosines are present in all multicellular eukaryotes, but unicellular eukaryotes such as yeast and prokaryotes are not capable of tyrosine sulfation.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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