Invitrogen™

HGFR (c-Met) Human ProcartaPlex™ Simplex Kit

The Human HGFR (c-Met) Simplex ProcartaPlex Kit measures HGFR (c-Met) protein and is designed to be combinable with other Simplex자세히 알아보기

The Human HGFR (c-Met) Simplex ProcartaPlex Kit measures HGFR (c-Met) protein and is designed to be combinable with other Simplex kits so that you can create your own multiplex panel that utilizes Luminex xMAP technology for protein detection/quantitation. When combining multiple Simplex kits (e.g., when you are not using a pre-configured multiplex panel), only one buffer kit (Cat. No. EPX010-10420-901) is needed for each assay plate regardless of plex size.

Recommended sample pre-dilution for serum and plasma samples: 1:100

About ProcartaPlex assays for the Luminex platform

ProcartaPlex immunoassays are based on the principles of a sandwich ELISA, using two highly specific antibodies binding to different epitopes of one protein to quantitate all protein targets simultaneously using a Luminex instrument. ProcartaPlex multiplex assays require as little as 25 μL of plasma or serum, or 50 μL of cell culture supernatant, and just four hours to obtain analyzed results.

• Reproducible, reliable results—validated as a panel to the highest industry standard, including protein target combinability and cross-reactivity testing
• More results per sample—measure multiple protein targets simultaneously in a single 25–50 μL sample
• Well-established Luminex technology—the most referenced multiplexing platform for protein detection and quantitation

ProcartaPlex assays utilize Luminex xMAP (multianalyte profiling) technology for the simultaneous detection and quantitation of up to 80 protein targets in a single 25–50 μL sample—from plasma, serum, cell culture supernatants, and other bodily fluids.

The Luminex MagPlex superparamagnetic microsphere beads in the ProcartaPlex assay are internally dyed with precise proportions of red and infrared fluorophores to create 100 spectrally unique signatures that can be identified by the Luminex xMAP detection systems (Luminex 200, FLEXMAP 3D, and MAGPIX systems). Similar to a sandwich ELISA, the ProcartaPlex assay uses matched antibody pairs to identify the protein of interest. In a multiplexed assay, each spectrally unique bead is labeled with antibodies specific for a single target protein, and bound proteins are identified with biotinylated antibodies and streptavidin–R-phycoerythrin (RPE). The conjugation of protein-specific antibodies to a distinct bead allows for analysis of multiple targets in a single well.

The most significant difference between a ProcartaPlex assay and ELISA is that the capture antibody in the ProcartaPlex assay is conjugated to a magnetic bead and not adsorbed to the microplate well, so the ProcartaPlex assay reagents are free-floating in the solution. For detection, the Luminex 200 instrument, for example, contains two lasers, one to distinguish the spectral signature of each bead and the second to quantify the amount of RPE fluorescence, which is proportional to the amount of protein present in the sample. ProcartaPlex multiplex assays can profile more target proteins using significantly less sample in the same time that it takes to perform a traditional sandwich ELISA.

ProcartaPlex multiplex panels are available in multiple formats across six species (human, mouse, rat, nonhuman primate, porcine, and canine). Visit thermofisher.com/procartaplex for more information and available products.

사양

제품 유형Simplex Kit

수량96 Tests

종Human

CombinabilityCombinable

비드 영역25

어세이 민감성See Certificate of Analysis

어세이 범위See Certificate of Analysis

형식Simplex Kit

용도(장비)Luminex™ Instruments

유전자MET

유전자 별칭HGFR, AUTS9, RCCP2, c-Met, DFNB97, HGF/SF receptor, HGF/SF

유전자 ID(Entrez)4233

유전자 기호MET

분석 간 CVLess than 15%

분석 내 CVLess than 15%

제품라인ProcartaPlex™

단백질Hepatocyte growth factor receptor

샘플 종류Serum, Plasma, Cell Culture Supernatants

샘플 부피Serum, Plasma: 25 μL, CCS: 50 μL

UniProt IDP08581

Research AreaOncology

Unit SizeEach