Thermo Fisher Scientific Claudin 1 Monoclonal Antibody (2H10D10)
Claudin-1 단백질의 C-말단 영역을 인식하는 Mouse IgG1 단클론 항체로, WB, IHC, ICC/IF 등 다양한 응용에 적합합니다. 사람, 개, 생쥐, 쥐 등 여러 종에 반응하며, Protein A로 정제된 액상 형태로 제공됩니다.
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Applications and Tested Dilutions
| Application | Tested Dilution | Publications |
|---|---|---|
| Western Blot (WB) | 1–3 µg/mL | 53 |
| Immunohistochemistry (IHC) | – | 14 |
| Immunohistochemistry (Paraffin) (IHC (P)) | Assay-dependent | 7 |
| Immunohistochemistry (PFA fixed) (IHC (PFA)) | – | 1 |
| Immunohistochemistry (Frozen) (IHC (F)) | 5–10 µg/mL | 1 |
| Immunocytochemistry (ICC/IF) | 1–5 µg/mL | 24 |
| Flow Cytometry (Flow) | – | 1 |
| ELISA | 0.1–1.0 µg/mL | – |
| Immunoprecipitation (IP) | 10 µg/test | – |
| Miscellaneous PubMed (Misc) | – | 16 |
Product Specifications
| Property | Description |
|---|---|
| Species Reactivity | Dog, Human, Mouse, Rat |
| Published Species | Chicken, Dog, Human, Mouse, Pig, Rabbit, Rat, Rhesus monkey |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Clone | 2H10D10 |
| Immunogen | Synthetic peptide derived from the C-terminal region of the human and mouse claudin-1 proteins |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 0.5 mg/mL |
| Purification | Protein A |
| Storage Buffer | PBS, pH 7.4 |
| Contains | 0.1% sodium azide |
| Storage Conditions | Refrigerate at 2–8°C up to 1 month; for long-term store at -20°C |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2533323 |
Additional Formats
Product Specific Information
Reactivity has been confirmed with human Caco-2 and canine MDCK cell lysates, mouse kidney and intestinal lysates, frozen mouse intestine (jejunum) tissue, and rat kidney homogenates.
This antibody is specific for the C-terminal region of the claudin-1 protein. On western blots, it identifies the target band at approximately 22 kDa.
Target Information
Claudin-1 is a member of the transmembrane protein family claudins located in cell-cell tight junctions and acts as a co-receptor for HCV entry into hepatic cells. Claudins are abundant in luminal epithelial sheets where they maintain epithelial cell polarity. Claudin-1 is expressed in most tissues such as bladder, fallopian tube, liver, pancreas, prostate, and skin.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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