Thermo Fisher Scientific PDPK1 Monoclonal Antibody (7E4G11)
PDPK1 단백질을 표적하는 Thermo Fisher Scientific의 단클론 항체로, Western blot, IHC, ICC/IF, Flow cytometry, ELISA 등 다양한 응용에 적합합니다. 인간, 생쥐, 설치류 등에서 반응하며, Protein G로 정제된 액상 형태로 제공됩니다. 연구용으로만 사용 가능합니다.
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Thermo Fisher Scientific PDPK1 Monoclonal Antibody (7E4G11)
Applications and Tested Dilutions
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:500–1:2,000 |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:200–1:1,000 |
| Immunocytochemistry (ICC/IF) | 1:50–1:200 |
| Flow Cytometry (Flow) | 1:200–1:400 |
| ELISA | 1:10,000 |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Human, Mouse, Non-human primate, Rat |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Clone | 7E4G11 |
| Immunogen | Purified recombinant fragment of human PDPK1 (AA: 457–556) expressed in E. coli |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Protein G |
| Storage Buffer | PBS |
| Contains | 0.05% sodium azide |
| Storage Conditions | Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles. |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2787457 |
Product Specific Information
MA5-31834 has been tested in indirect ELISA.
Target Information
PDPK1 (3-Phosphoinositide Dependent Protein Kinase 1) phosphorylates AGC kinases and activates conventional PKC and PKC zeta through phosphorylation of critical threonine residues in the activation loop.
It also phosphorylates Protein Kinase B (PKB) at threonine 308 in the presence of phosphatidylinositol-3,4,5-trisphosphate. Active Akt inactivates Glycogen Synthase Kinase 3 (GSK3), leading to dephosphorylation and activation of glycogen synthase, and stimulation of glycogen synthesis.
Because of its role in insulin-induced glycogen synthesis and PKC activation, PDPK1 is a potentially important target for metabolic drug research.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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