Thermo Fisher Scientific ATG16L1 Monoclonal Antibody (OTI2A10), TrueMAB
ATG16L1 단백질을 인식하는 단클론 항체로, Western blot에 적합합니다. Human, Mouse, Rat에 반응하며, IgG1 아이소타입입니다. 동결건조 형태로 제공되며, PBS 버퍼에 8% trehalose 포함. 오토파지 연구용으로 적합합니다.
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Applications
Western Blot (WB)
- Tested Dilution: 1:2,000
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human, Mouse, Rat |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Clone | OTI2A10 |
| Immunogen | Human recombinant protein fragment corresponding to amino acids 1–277 of human ATG16L produced in E. coli |
| Conjugate | Unconjugated |
| Form | Lyophilized |
| Concentration | 1 mg/mL |
| Purification | Affinity chromatography |
| Storage buffer | PBS, pH 7.3, with 8% trehalose |
| Contains | No preservative |
| Storage conditions | -20°C, Avoid Freeze/Thaw Cycles |
| Shipping conditions | Ambient (domestic); Wet ice (international) |
Product Specific Information
For reconstitution, add 100 µL distilled water to obtain a final antibody concentration of approximately 1 mg/mL.
For conjugation experiments, perform an additional desalting step using Zeba Spin Desalting Columns (7K MWCO, 0.5 mL, Product #89882).
Target Information
Autophagy related protein 16L (encoded by ATG16L1, located on chromosome 2 in humans) is also known as WD30 or IBD10 (Inflammatory Bowel Disease 10).
ATG12 and ATG5 conjugate and form a complex with ATG16L (approximately 800 kDa), known as the Atg16L complex, which facilitates the lipidation of LC3 to phosphatidylethanolamine (PE)—a key marker for autophagy.
This complex also prevents premature cleavage of ATG8-PE by Atg4 until autophagosome completion.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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