Thermo Fisher Scientific ATG16L1 Monoclonal Antibody (OTI5A2), TrueMAB
ATG16L1 단백질을 표적하는 단클론 항체로 Western blot 및 IHC에 최적화되어 있습니다. Human, Mouse, Rat 반응성을 가지며, carrier-free 형태로 제공되어 결합 실험에도 적합합니다. 고순도 친화 크로마토그래피 정제 및 안정적인 동결건조 형태로 보관 가능합니다.
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Applications
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:500 |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:2,000 |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Mouse, Rat, Human |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Clone | OTI5A2 |
| Immunogen | Human recombinant protein fragment corresponding to amino acids 1–277 of human ATG16L1 produced in E. coli |
| Conjugate | Unconjugated |
| Form | Lyophilized |
| Concentration | 1 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.3, with 8% trehalose |
| Contains | No preservative |
| Storage Conditions | -20°C, Avoid Freeze/Thaw Cycles |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Product Specific Information
For reconstitution, add 100 µL distilled water to achieve a final antibody concentration of approximately 1 mg/mL.
For conjugation experiments, perform an additional desalting step using Zeba Spin Desalting Columns (7K MWCO, 0.5 mL, Product #89882).
Target Information
Autophagy related protein 16L1 (ATG16L1) is encoded by the ATG16L gene located on chromosome 2 in humans. Also known as WD30 or IBD10, it forms a complex with ATG12 and ATG5 to generate the Atg16L complex (~800 kDa). This complex facilitates LC3 lipidation with phosphatidylethanolamine (PE), a key marker for autophagy.
ATG16L1, together with ATG18 and ATG21, prevents premature cleavage of ATG8-PE by the cysteine protease Atg4 until autophagosome completion.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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