Thermo Fisher Scientific PPAR alpha Monoclonal Antibody (3B6/PPAR)

Applications

Tested Dilution

Publications

Western Blot (WB)

2-5 µg/mL

View 16 publications 16 publications

Immunohistochemistry (IHC)

-

View 4 publications 4 publications

Immunocytochemistry (ICC/IF)

2 µg/mL

View 1 publication 1 publication

Flow Cytometry (Flow)

1 µg / 10^6 cells

-

Immunoprecipitation (IP)

Assay-dependent

View 2 publications 2 publications

ChIP assay (ChIP)

Assay-dependent

View 1 publication 1 publication

Gel Shift (GS)

Assay-dependent

-

Product Specifications

Species Reactivity

Human, Mouse, Rat

Published species

Bovine, Human, Mouse, Rabbit, Rat

Host/Isotype

Mouse / IgG2b

Class

Monoclonal

Type

Antibody

Clone

3B6/PPAR

Immunogen

Purified recombinant PPAR alpha protein.

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

1 mg/mL

Purification

purified

Storage buffer

PBS

Contains

0.05% sodium azide

Storage conditions

-20° C, Avoid Freeze/Thaw Cycles

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_2165745

Product Specific Information

MA1-822 detects peroxisome proliferator activated receptors (PPAR) alpha from human, mouse, and rat tissues.

MA1-822 has been successfully used in Western blot, Gel Shift and immunoprecipitation procedures. By Western blot, this antibody detects a ~52 kDa protein which corresponds to PPAR alpha from mouse adipose tissue extract. This antibody detects some non-specific bands on NIH-3T3 cell lysates.

The MA1-822 immunogen is purified recombinant PPAR alpha protein.

Target Information

Peroxisome proliferators are non-genotoxic carcinogens which are purported to exert their effect on cells through their interaction with members of the nuclear hormone receptor family termed peroxisome proliferator activated receptors (PPARs). Nuclear hormone receptors are ligand-dependent intracellular proteins that stimulate transcription of specific genes by binding to specific DNA sequences following activation by the appropriate ligand. Studies indicate that PPARs are activated by peroxisome proliferators such as clofibric acid, nafenopin, and WY-14,643, as well as by some fatty acids. It has also been shown that PPAR`s can induce transcription of acyl coenzyme A oxidase & cytochrome P450 (CYP450) A6 through interaction with specific response elements. PPAR, like several other nuclear hormone receptors, heterodimerizes with retinoid X receptor (RXR) alpha.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.