Thermo Fisher Scientific HLA-B7 Monoclonal Antibody (BB7.1), FITC

Applications

Tested Dilution

Publications

Flow Cytometry (Flow)

Neat

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Product Specifications

Species Reactivity

Cynomolgus monkey, Human

Published species

Human

Host/Isotype

Mouse / IgG1

Class

Monoclonal

Type

Antibody

Clone

BB7.1

Immunogen

Papain solubilized HLA-B7 antigen.

Conjugate

FITC FITC FITC

View additional formats

• Unconjugated
• PE
• Request custom conjugation

Excitation/Emission Max

498/517 nm View spectra

Form

Liquid

Concentration

0.1 mg/mL

Purification

Protein A

Storage buffer

PBS, pH 7.4, with 1% BSA

Contains

0.09% sodium azide

Storage conditions

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles. Store in the dark.

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_931644

Product Specific Information

For FACS analysis, use 10 µL of the suggested working dilution to label 1x10^6 cells in 100 µL.

Mouse anti Human HLA B7 antibody, clone BB7.1 recognizes the HLA B7 antigen and does not cross-react with HLA B27 or other related antigens.

Target Information

HLA-B7 is one of the polymorphic proteins of human MHC Class I found on all nucleated cells. This surface protein is a heterodimer consisting of the specific transmembrane HLA-B chain paired with Beta-2 microglobulin. Classical MHC Class I molecules play a central role in adaptive immunity by presenting to T cells antigenic peptides derived either from the cell itself (classical presentation) or extracellular antigens (cross-presentation). The ligands of MHC class I include T cell receptor (TCR) and a costimulatory molecule CD8. Hundreds of HLA-B alleles have been described. Typing for these polymorphisms is routinely done for transplantation.

HLA and MHC antibodies play a significant role in Immunopeptidomics, facilitating the identification and characterization of neoantigens through high-performance liquid chromatography coupled to tandem Mass Spectrometry.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.