Thermo Fisher Scientific CD205 Monoclonal Antibody (205yekta), NovaFluor Red 685, eBioscience

Thermo Fisher Scientific CD205 Monoclonal Antibody (205yekta), NovaFluor Red 685, eBioscience

Applications and Tested Dilution

• Application: Flow Cytometry (Flow)
• Tested Dilution: 0.2 µg/test

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Product Specifications

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Additional Formats Available

• Alexa Fluor 488
• APC
• Biotin
• NovaFluor Blue 660-120S
• NovaFluor Red 700
• NovaFluor Red 755
• NovaFluor Yellow 610
• NovaFluor Yellow 660
• NovaFluor Yellow 700
• NovaFluor Yellow 755
• PE
• PE-Cyanine7
• PE-eFluor 610
• PerCP-eFluor 710
• Super Bright 436
• Super Bright 600
• Super Bright 645
• Request custom conjugation

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Product Specific Information

Description:
The 205yekta antibody reacts with mouse CD205 (DEC-205), an integral membrane glycoprotein involved in antigen uptake, trafficking, and presentation that enhances antigen-specific T cell immunity. CD205 is highly expressed by CD8+ dendritic cells and also found in bone marrow Gr1+ cells, Langerhans cells, BMDCs, and thymic epithelial cells.

CD205 binds to PE-Cy5.5 antibody and streptavidin conjugates; therefore, this format should not be used on CD205+ dendritic cells.

Each product contains:

• 1 vial of NovaFluor conjugate
• 1 vial of CellBlox Plus Blocking Buffer

Applications Reported: Flow cytometric analysis
Applications Tested: Flow cytometric analysis of mouse bone marrow cells (≤0.2 µg/test, 100 µL final volume). Cell number: 10⁵–10⁸ cells/test. Antibody titration recommended for optimal performance.

Compatibility Notes:
NovaFluor dyes are not compatible with DNA intercalating viability dyes (e.g., propidium iodide, 7-AAD, DAPI). Use Invitrogen LIVE/DEAD Fixable Dead Cell stains with NovaFluor dyes.

Blocking Buffer Recommendation:
Use CellBlox Plus Blocking Buffer (Cat. No. C001T06F01) for optimal staining.

• Add 5 µL per sample before combining with cells.
• For single-color controls: 5 µL per 100 µL cell sample (10³–10⁸ cells).

Technology:
NovaFluor conjugates are based on Phiton™ technology utilizing nucleic acid dye structures engineered for optimized fluorescent signatures with minimal spillover and spread.

Excitation: 637 nm
Emission: 685 nm
Laser: 633–640 nm (Red)

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Target Information

CD205 (LY25, Lymphocyte Antigen 75) is an integral membrane glycoprotein involved in antigen uptake, trafficking, and presentation, enhancing antigen-specific T cell immunity. It is highly expressed by CD8+ dendritic cells and other immune cell types. CD205 acts as an endocytic receptor directing captured antigens to specialized antigen processing regions and may reduce B lymphocyte proliferation.
Associated diseases include pneumonic plague and lipid pneumonia.

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For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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