Thermo Fisher Scientific Phospho-RSK Pan (Ser221, Ser227, Ser218, Ser232) Recombinant Superclonal Antibody

Applications

Tested Dilution

Publications

Western Blot (WB)

1-2 µg/mL

-

Product Specifications

Species Reactivity

Human

Host/Isotype

Rabbit / IgG

Expression System

Expi293

Class

Recombinant Superclonal

Type

Antibody

Immunogen

A synthesized peptide derived from human RSK1/2/3/4 around the phosphorylation site of Ser221/227/218/223 if (typeof window.$mangular === undefined || !window.$mangular) { window.$mangular = {}; } $mangular.antigenJson = \[{targetFamily:RSK Pan,uniProtId:Q15349-1,ncbiNodeId:9606,antigenRange:218,antigenLength:733,antigenImageFileName:711336_RSK_Pan_Q15349-1_Rabbit.svg,antigenImageFileNamePDP:711336_RSK_Pan_Q15349-1_Rabbit_PDP.jpeg,sortOrder:1},{targetFamily:RSK Pan,uniProtId:Q9UK32-1,ncbiNodeId:9606,antigenRange:232,antigenLength:745,antigenImageFileName:711336_RSK_Pan_Q9UK32-1_Rabbit.svg,antigenImageFileNamePDP:711336_RSK_Pan_Q9UK32-1_Rabbit_PDP.jpeg,sortOrder:2},{targetFamily:RSK Pan,uniProtId:Q15418-1,ncbiNodeId:9606,antigenRange:221,antigenLength:735,antigenImageFileName:711336_RSK_Pan_Q15418-1_Rabbit.svg,antigenImageFileNamePDP:711336_RSK_Pan_Q15418-1_Rabbit_PDP.jpeg,sortOrder:3},{targetFamily:RSK Pan,uniProtId:P51812-1,ncbiNodeId:9606,antigenRange:227,antigenLength:740,antigenImageFileName:711336_RSK_Pan_P51812-1_Rabbit.svg,antigenImageFileNamePDP:711336_RSK_Pan_P51812-1_Rabbit_PDP.jpeg,sortOrder:4}\]; $mangular.isB2BCMGT = false; $mangular.isEpitopesModalImageMultiSizeEnabled = true;

View immunogen .st0{fill:#FFFFFF;} .st1{fill:#1E8AE7;}

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.5 mg/mL

Purification

Protein A

Storage buffer

PBS, pH 7.4

Contains

0.09% sodium azide

Storage conditions

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Shipping conditions

Wet ice

RRID

AB_2633010

Product Specific Information

This antibody is predicted to react with Monkey, Rat and Mouse

Recombinant rabbit Superclonal™ antibodies are unique offerings from Thermo Fisher Scientific. They are comprised of a selection of multiple different recombinant monoclonal antibodies, providing the best of both worlds - the sensitivity of polyclonal antibodies with the specificity of monoclonal antibodies - all delivered with the consistency only found in a recombinant antibody. While functionally the same as a polyclonal antibody - recognizing multiple epitope sites on the target and producing higher detection sensitivity for low abundance targets - a recombinant rabbit Superclonal™ antibody has a known mixture of light and heavy chains. The exact population can be produced in every lot, circumventing the biological variability typically associated with polyclonal antibody production. Note: Formerly called “Recombinant polyclonal antibody”, this product is now rebranded as “Recombinant Superclonal™ antibody”. The physical product and the performance remain unchanged.

Target Information

The p90 ribosomal S6 kinases (RSKs) comprise a family of serine/threonine kinases that lie at the terminus of the ERK pathway. In humans, the RSK family consists of four isoforms (RSK1 to-4). RSK family members are unusual among serine/threonine kinases in that they contain two distinct kinase domains, both of which are catalytically functional. Theses kinase domains are activated in a sequential manner by a series of phosphorylations. RSK regulates gene expression via association and phosphorylation of transcriptional regulators including c-Fos, estrogen receptor, NFkappaB/IkappaB alpha, cAMP-response element-binding protein (CREB). ERK activates the C-terminal kinase of RSK, leading to activation of the N-terminal kinase. Members of the RSK family are present in the cytoplasm as well as the nucleus. Addition of growth factor to the cells results in the activation of both cytosolic and nuclear RSK and the translocation of the cytosolic RSK into the nucleus upon activation. The activation and nuclear translocation of RSK result in phosphorylation and activation of transcription factors.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.