Thermo Fisher Scientific Integrin beta 7 Monoclonal Antibody (FIB504), PE-Cyanine7, eBioscience

Thermo Fisher Scientific Integrin beta 7 Monoclonal Antibody (FIB504), PE-Cyanine7, eBioscience

Applications

• Flow Cytometry (Flow)

Tested Dilution

• 5 µL (0.125 µg)/test

Publications

• View 3 publications

Product Specifications

Additional Formats

• Unconjugated
• Biotin
• FITC
• PE
• Request custom conjugation

Product Specific Information

Description:
The FIB504 monoclonal antibody reacts with human and mouse integrin beta 7. Integrin beta 7 is a 130 kD glycoprotein that associates with integrin alpha 4 (CD49d) to form the alpha 4 beta 7 integrin LPAM-1, expressed on intraepithelial lymphocytes. It also associates with alpha E (CD103) to form the alpha E beta 7 integrin HML-1, expressed on T cells adjacent to mucosal epithelium and intraepithelial lymphocytes.
Main ligands for integrin alpha 4 beta 7 include VCAM-1 (CD106), MAdCAM-1, and fibronectin, while the main ligand of integrin alpha E beta 7 is E-cadherin (CD324).
Integrin beta 7 plays an important role in leukocyte adhesion and transmigration during inflammatory responses. The FIB504 antibody has been reported to have blocking activity.

Applications Reported:
This antibody has been reported for use in flow cytometric analysis.

Applications Tested:
Pre-titrated and tested by flow cytometric analysis of normal human peripheral blood cells. Use at 5 µL (0.125 µg) per test (100 µL final volume). Cell number: 10⁵–10⁸ cells/test (empirically determined).

Light Sensitivity:
This tandem dye is sensitive to photo-induced oxidation. Protect vial and stained samples from light.

Fixation:
Samples can be stored in IC Fixation Buffer (Product # 00-822-49) or 1-step Fix/Lyse Solution (Product # 00-5333-54) for up to 3 days at 4°C in the dark with minimal impact on brightness and FRET efficiency.

Excitation/Emission:
Excitation 488–561 nm; Emission 775 nm; compatible lasers: Blue, Green, Yellow-Green.

Filtration:
0.2 µm post-manufacturing filtered.

Target Information

Integrin beta 7 associates with integrin alpha 4 (CD49d) to form LPAM-1 and with alpha E (CD103) to form HML-1. These complexes mediate adhesion and transmigration of leukocytes via interactions with VCAM-1, MAdCAM-1, fibronectin, and E-cadherin. Plays a key role in mucosal immunity and inflammation.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

제품 이미지