Thermo Fisher Scientific CD369 (Clec7a, Dectin-1) Monoclonal Antibody (bg1fpj), PE-Cyanine7, eBioscience

Applications and Tested Dilution

Product Specifications

Additional Formats

• APC
• PE
• PerCP-eFluor 710
• Request custom conjugation

Product Specific Information

The monoclonal antibody bg1fpj recognizes mouse CD369 (Dectin-1, CLEC7A), a type II transmembrane protein in the C-type lectin family. Its ligand is β1,3-glucan, a key polysaccharide component in the cell wall of fungi, plants, and some bacteria.
CD369 binds organisms such as Candida, Aspergillus, Pneumocystis, Coccidioides, and Mycobacterium. Upon ligand binding, calcium-dependent signaling via the ITAM domain activates Syk kinase, leading to endocytosis/phagocytosis and cytokine production (IL-2, IL-6, IL-10, TNF-α, IL-1β, IL-23).
CD369 signaling contributes to Th17 and Th1 differentiation and is mainly expressed on myeloid cells (neutrophils, monocytes, macrophages, dendritic cells), with lower expression on lymphocytes and mucosal epithelium.

Applications Reported: Flow cytometric analysis.
Applications Tested: Tested by flow cytometric analysis of mouse lysed whole blood. Recommended ≤0.125 µg/test (100 µL final volume). Optimize empirically per assay (10⁵–10⁸ cells/test).
Light Sensitivity: Protect from light; tandem dye is sensitive to photo-induced oxidation.
Fixation: Samples can be stored up to 3 days at 4°C in the dark using:

• IC Fixation Buffer (Product #00-8222-49)
• 1-step Fix/Lyse Solution (Product #00-5333-54)
  Fluorophore performance may vary by clone; confirm empirically.
• Excitation/Emission:* 488–561 nm / 775 nm
• Laser Compatibility:* Blue, Green, Yellow-Green
• Filtration:* 0.2 µm post-manufacturing filtered.

Target Information

CLEC7A (Dectin-1) is a member of the C-type lectin-like receptor family, predominantly expressed on myeloid cells. It is a small type II membrane glycoprotein with an extracellular C-type lectin-like domain and a cytoplasmic ITAM motif.
CLEC7A functions as a pattern-recognition receptor that detects β-1,3- and β-1,6-linked glucans from fungi and plants, triggering innate immune responses through Syk kinase activation and reactive oxygen species production.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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