Thermo Fisher Scientific TCR V alpha 2 Monoclonal Antibody (B20.1), PE-Cyanine7, eBioscience
마우스 TCR V alpha 2 체인을 인식하는 B20.1 단클론 항체로, PE-Cyanine7 형광체가 결합되어 있습니다. Flow Cytometry에 최적화되어 있으며, 0.125 µg/test로 사용 가능합니다. 광민감성이 있으므로 암조건에서 보관하며, 4°C 보관 권장.
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Thermo Fisher Scientific TCR V alpha 2 Monoclonal Antibody (B20.1), PE-Cyanine7, eBioscience
Applications and Tested Dilution
- Application: Flow Cytometry (Flow)
- Tested Dilution: 0.125 µg/test
- Publications: View 2 publications
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Mouse |
| Published Species | Not Applicable |
| Host / Isotype | Rat / IgG2a, lambda |
| Recommended Isotype Control | Rat IgG2a kappa Isotype Control (eBR2a), PE-Cyanine7, eBioscience™ |
| Class | Monoclonal |
| Type | Antibody |
| Clone | B20.1 |
| Conjugate | PE-Cyanine7 |
| Excitation/Emission Max | 569 / 780 nm |
| Form | Liquid |
| Concentration | 0.2 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.2 |
| Contains | 0.09% sodium azide |
| Storage Conditions | 4°C, store in dark, DO NOT FREEZE! |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2573472 |
Available Formats
- APC
- APC-eFluor 780
- Biotin
- eFluor 450
- FITC
- NovaFluor Blue 610-70S
- NovaFluor Red 700
- NovaFluor Yellow 570
- NovaFluor Yellow 610
- NovaFluor Yellow 660
- PE
- PerCP-eFluor 710
- Super Bright 436
- Super Bright 600
- Super Bright 780
- Request custom conjugation available
Product Specific Information
Description:
The B20.1 monoclonal antibody reacts with mouse T-cell receptor (TCR) V alpha 2 chains. It recognizes the majority of the TCR V alpha 2 subfamily in mice carrying the a, b, and c haplotypes and also reacts with V delta 8 products due to high homology.
Applications Reported:
Reported for use in flow cytometric analysis.
Applications Tested:
Tested by flow cytometry using mouse splenocytes. Recommended ≤0.125 µg per test (100 µL final volume). Optimal titration is advised for best assay performance.
Light Sensitivity:
This tandem dye is sensitive to photo-induced oxidation. Protect vial and stained samples from light.
Fixation:
Samples can be stored in IC Fixation Buffer (Product #00-8222-49) or 1-step Fix/Lyse Solution (Product #00-5333-54) for up to 3 days at 4°C in the dark with minimal impact on brightness and FRET efficiency.
Excitation/Emission:
Excitation: 488–561 nm
Emission: 775 nm
Laser: Blue, Green, Yellow-Green
Filtration:
0.2 µm post-manufacturing filtered.
Target Information
T-cell receptors (TCR) discriminate foreign from self-peptides presented by MHC class II molecules, essential for adaptive immune response. Recognition of self-peptides is linked to autoimmune diseases and tumor-associated mutant peptides. Superantigen engagement can lead to toxic shock syndrome. Autoantibodies to V beta TCR segments are found in RA and SLE patients, potentially modulating immune activity. Most human T cells express TCR alpha-beta with CD4 or CD8, while gamma-delta T cells are found in epithelia with distinct antigen recognition and immunoregulatory roles.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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