Thermo Fisher Scientific FAS (CD95) Chimeric Recombinant Rabbit Monoclonal Antibody (R-125224)

Applications and Tested Dilutions

Product Specifications

Product Specific Information

Specificity:
R-125224 binds to the extracellular portion of human Fas at an epitope consisting of the sequence RTQNTKCRCK (aa 105–114).

Preparation:
R-125224 is generated by the humanization of the murine HFE7A anti-Fas antibody by grafting the CDR regions to the framework regions of the human 8E10 antibody and substituting key framework residues from the murine antibody into the 8E10 sequence. The original HFE7A was derived from a hybridoma cell line generated by the fusion of NS1 myeloma cells with splenocytes from Fas-deficient mice immunized with partially purified recombinant human Fas-AIC2A chimera protein. The HFE7A hybridoma was selected after screening by flow cytometry for antibodies binding to WR19L12a or L5178YA1 cells expressing human or murine Fas, but not to the parental cell lines.

Target Information

FAS (Fas, CD95, APO-1) is a 46 kDa transmembrane glycoprotein functioning as a cell death receptor of the TNFR superfamily. It also exists in a soluble form (~26 kDa). Stimulation of FAS results in aggregation of intracellular FAS-associated death domains (FADD), formation of the death-inducing signaling complex (DISC), and activation of caspases. This cascade leads to apoptosis. FAS can activate NF-kappaB, MAPK3/ERK1, and MAPK8/JNK, and is involved in proliferative signaling in fibroblasts and T cells. Multiple alternatively spliced transcript variants exist, with some isoforms lacking the transmembrane domain acting as negative regulators of apoptosis. The Fas/Fas ligand system is implicated in diseases such as AIDS, hepatitis, and cancer, and plays a key role in antiviral immune responses.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.