Thermo Fisher Scientific TNF alpha Monoclonal Antibody (45418)

Applications

Tested Dilution

Publications

Western Blot (WB)

1 µg/mL

-

ELISA (ELISA)

0.1-0.4 µg/mL (Detection), 2-8 µg/mL (Capture)

-

Neutralization (Neu)

10-40 µg/mL

-

Product Specifications

Species Reactivity

Rat

Host/Isotype

Mouse / IgG1

Class

Monoclonal

Type

Antibody

Clone

45418

Immunogen

E. coli-derived recombinant rat TNF-alpha

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Lyophilized

Concentration

0.5 mg/mL

Purification

Protein A/G

Storage buffer

PBS with 5% trehalose

Contains

No Preservative

Storage conditions

-20° C, Avoid Freeze/Thaw Cycles

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_2609357

Product Specific Information

In ELISAs, this antibody shows less than 3% cross-reactivity with recombinant mouse (rm) TNF- alpha and less than 0.2% cross-reactivity with rhTNF- alpha, rpTNF- alpha, and rhTNF- beta. In Western blots, this antibody shows 100% cross-reactivity with rmTNF- alpha and no cross-reactivity with rfeTNF- alpha.

Reconstitute at 0.5 mg/mL in sterile PBS.

Endoxin level is <0.10 EU per 1 µg of the antibody by the LAL method.

Target Information

TNF alpha is a multifunctional proinflammatory cytokine that belongs to the tumor necrosis factor (TNF) superfamily. This cytokine is mainly secreted by macrophage and bind to its receptors, TNFRSF1A/TNFR1 and TNFRSF1B/TNFBR. TNF alpha is involved in the regulation of immune cells, cell proliferation, differentiation, apoptosis, lipid metabolism, and coagulation. TNF alpha exists as a multimer of two, three, or five noncovalently linked units, but shows a single 17 kDa band following SDS-PAGE under non-reducing conditions. Knockout studies in mice also suggested the neuroprotective function of TNF alpha, and has been observed to causes tumor necrosis when injected into tumor-bearing mice. Other functions of TNF-alpha include its role in the immune response to bacterial, viral, parasitic and certain fungal infections, as well as its role in the necrosis of specific tumors. TNF alpha causes cytolysis or cytostasis of certain transformed cells, being synergistic with interferon-gamma in its cytotoxicity. This cytokine has been implicated in a variety of diseases, including autoimmune diseases, insulin resistance, and cancer.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.