Thermo Fisher Scientific NK1.1 Monoclonal Antibody (PK136), Super Bright 780, eBioscience

Applications and Tested Dilution

Product Specifications

Additional Available Formats

Unconjugated, Alexa Fluor 561, Alexa Fluor 660, Alexa Fluor 700, APC, APC-eFluor 780, Biotin, Brilliant UV 395, Brilliant UV 496, Brilliant UV 615, Brilliant UV 661, Brilliant UV 805, Brilliant Violet 421, Brilliant Violet 480, Brilliant Violet 605, Brilliant Violet 650, Brilliant Violet 711, Brilliant Violet 786, eFluor 450, FITC, Functional Grade, PE, PE-Cyanine7, PE-eFluor 610, PerCP-Cyanine5.5, Super Bright 436, Super Bright 600, Super Bright 645, Super Bright 702.
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Product Specific Information

The PK136 monoclonal antibody reacts with mouse NK1.1, an antigen expressed by natural killer cells and a subset of T cells in NK1.1 mouse strains such as C57BL and NZB.
Common laboratory strains like BALB/c, SJL, AKR, CBA, C3H, and A do not express NK1.1. For these, use the DX5 (14-5971) antibody.
Simultaneous staining of C57BL/6 spleen cells with PK136 and DX5 shows coexpression in most cells, with smaller DX5⁺PK136⁻ and DX5⁻PK136⁺ populations.

• Applications Reported: Flow cytometric analysis
• Applications Tested: Validated for ≤0.5 µg/test in flow cytometry using C57Bl/6 splenocytes
• Excitation: 405 nm
• Emission: 780 nm
• Laser: Violet laser

Super Bright 780 is a tandem dye excited by 405 nm and emits at 780 nm (recommended 780/60 bandpass filter).
Use Super Bright Complete Staining Buffer (SB-4401) when using multiple Super Bright dyes to minimize non-specific interactions.

Compensation Considerations:
When using UltraComp eBeads (01-2222-42) or AbC Total Antibody Compensation beads (A10497), higher compensation values may occur in the violet 450/50 channel. Setting compensation with cells is recommended.

Light Sensitivity:
Protect from light to avoid photo-induced oxidation.

Fixation:
Samples can be stored in IC Fixation Buffer (00-8222) or 1-step Fix/Lyse Solution (00-5333) for up to 3 days at 4°C in the dark with minimal effect on brightness or FRET efficiency. Clone-specific performance should be determined empirically.

Super Bright Polymer Dyes are sold under license from Becton, Dickinson and Company.

Target Information

NK1.1 (KLRB1, NKR-P1A, CD161)
NK1.1 is a marker for natural killer (NK) cells and some T cell subsets. It belongs to the C-type lectin superfamily and plays a role in NK cell regulation.
The KLRB1 protein is a type II membrane protein with an extracellular C-type lectin domain, transmembrane region, and cytoplasmic tail.
Its receptor, NKR-P1A (CD161), recognizes Lectin-Like Transcript-1 (LLT1) as a functional ligand.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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