Thermo Fisher Scientific IL-9 Monoclonal Antibody (MH9D1), PE, eBioscience
인간 IL-9을 인식하는 MH9D1 단클론 항체로, PE 형광 표지되어 유세포분석(Flow Cytometry)에 최적화됨. Th9 세포 연구 및 IL-9 관련 면역반응 분석에 적합. 4°C 보관, 동결 금지. 연구용 전용 시약.
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Applications
- Flow Cytometry (Flow)
Tested Dilution: 5 µL (0.06 µg)/test
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Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human |
| Published Species | Human |
| Host / Isotype | Mouse / IgG2a, kappa |
| Recommended Isotype Control | Mouse IgG2a kappa Isotype Control (eBM2a), PE, eBioscience™ |
| Class | Monoclonal |
| Type | Antibody |
| Clone | MH9D1 |
| Conjugate | PE |
| Excitation / Emission Max | 565 / 576 nm |
| Form | Liquid |
| Concentration | 5 µL/Test |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.2, with 0.2% BSA |
| Contains | 0.09% sodium azide |
| Storage Conditions | 4°C, store in dark, DO NOT FREEZE |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_11151145 |
Additional Formats
Product Specific Information
The monoclonal antibody MH9D1 recognizes interleukin-9 (IL-9), a proinflammatory cytokine involved in immune regulation. IL-9 is secreted by Th9 cells, which can be derived from Th2 cells with TGF-β or directly from naïve CD4+ T cells with TGF-β and IL-4.
IL-9 belongs to the common cytokine receptor γ-chain-dependent family, including IL-2, IL-4, IL-7, IL-15, and IL-21. It affects Th2 lymphocytes, B lymphocytes, mast cells, eosinophils, and epithelial cells, and is implicated in asthma and allergy-related disorders.
Staining with MH9D1 is not blocked by MH9A4, indicating distinct epitope recognition.
Applications Reported: Intracellular staining followed by flow cytometric analysis.
Applications Tested: Pre-titrated and validated on restimulated Th2-polarized human PBMCs using the Intracellular Fixation & Permeabilization Buffer Set (Product #88-8824-00).
Refer to BestProtocols®:
Protocol A – Two-step protocol for cytoplasmic intracellular proteins.
Recommended usage: 5 µL (0.06 µg)/test in a 100 µL staining volume.
Applicable for 10⁵–10⁸ cells/test (empirically determined).
Excitation: 488–561 nm
Emission: 578 nm
Laser Compatibility: Blue, Green, Yellow-Green
Filtration: 0.2 µm post-manufacturing filtered
Target Information
Interleukin 9 (IL-9) is produced by activated Th2 lymphocytes and regulates multiple cell types involved in asthma-related immune responses, including B/T lymphocytes, mast cells, eosinophils, and epithelial cells.
Mouse IL-9 cDNA encodes a 144 amino acid precursor with an 18 amino acid signal peptide, forming a mature 14 kDa glycosylated protein.
TGF-β plays a key role in Th9 differentiation by reprogramming Th2 cells to secrete IL-9.
For Research Use Only.
Not for use in diagnostic procedures or resale without authorization.
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