Thermo Fisher Scientific CD154 (CD40 Ligand) Monoclonal Antibody (MR1), Functional Grade, eBioscience

Applications and Tested Dilutions

Product Specifications

Available Formats

• Unconjugated
• Alexa Fluor 488
• APC
• Biotin
• eFluor 450
• PE
• PE-Cyanine7
• PE-eFluor 610
• PerCP-eFluor 710
• Super Bright 436
• Super Bright 600
• Request custom conjugation

Product Specific Information

Description:
The MR1 monoclonal antibody reacts with mouse CD154, a 39 kDa transmembrane glycoprotein also known as CD40 ligand (CD40L). CD154 is expressed transiently by activated T cells. Through its binding to CD40 on antigen-presenting cells (APCs) including B cells, monocytes/macrophages, and dendritic cells, it plays a key role in T cell–APC interactions. CD154–CD40 binding transduces signals for T-dependent B cell activation and induces B cells to enter the cell cycle.

For flow cytometric staining, it is recommended to stimulate enriched T cells or CD4+ cells before staining with MR1.

Applications Reported:
The MR1 antibody is reported for use in flow cytometric analysis and for inhibiting CD154–CD40 binding and B cell activation.

Applications Tested:
Tested by flow cytometric analysis of stimulated mouse splenocytes. Recommended ≤0.5 µg per test (100 µL final volume). Optimize antibody titration for best results.

Storage and Handling:
Use in a sterile environment.

Filtration:
0.2 µm post-manufacturing filtered.

Purity:

90% (SDS-PAGE)

Endotoxin Level:
<0.001 ng/µg antibody (LAL assay)

Aggregation:
<10% (HPLC)

Target Information

CD40 Ligand (CD40-L, CD154) is a membrane glycoprotein and differentiation antigen expressed on T cells. It stimulates B cell proliferation and immunoglobulin secretion in the presence of cytokines. CD40 Ligand also induces cytokine production and tumoricidal activity in monocytes, and co-stimulates activated T cell proliferation with IFN-γ, TNF-α, and IL-2 production.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.