Thermo Fisher Scientific CD150 Monoclonal Antibody (9D1), Functional Grade, eBioscience

Thermo Fisher Scientific CD150 Monoclonal Antibody (9D1), Functional Grade, eBioscience™

Applications and Tested Dilution

• Flow Cytometry (Flow): 1 µg/test
• Functional Assay: Assay-dependent

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Product Specifications

Available Conjugate Formats

• APC
• Biotin
• FITC
• PE
• PE-Cyanine7
• Request custom conjugation

Product Specific Information

The 9D1 monoclonal antibody reacts with mouse CD150, a ~70 kDa transmembrane glycoprotein also known as Signaling Lymphocyte Activation Molecule (SLAM).
CD150 is expressed by T (especially TH1) and B cells and is rapidly upregulated upon activation. Immature thymocytes and dendritic cells also express this antigen.
Signaling through SLAM induces T-cell proliferation and enhances interferon-gamma response. SLAM also mediates adhesion between T cells and antigen-presenting cells.

Mouse hematopoietic stem cells (HSC) can be identified using SLAM family markers such as CD150⁺CD244⁻CD48⁻. For this application, antibody clone mShad150 is recommended (Product #12-1502).

Applications Reported:

• Flow cytometric analysis
• Augmentation of T-cell activation (proliferation and IFN-γ production)

Applications Tested:

• Flow cytometry of mouse thymic and splenic cells
• ≤1 µg/test (100 µL final volume, 10⁵–10⁸ cells/test)
• Optimize antibody titration for best performance

Storage and Handling:

• Use in sterile environment
• 0.2 µm post-manufacturing filtration

Purity: >90% (SDS-PAGE)
Endotoxin Level: <0.001 ng/µg (LAL assay)
Aggregation: <10% (HPLC)

Target Information

CD150 (SLAM, Signaling Lymphocyte Activation Molecule) belongs to the immunoglobulin gene superfamily and is involved in T cell stimulation.
It is expressed on memory T cells, thymocytes, and subsets of B cells, and is rapidly induced on naïve T cells after activation.
Activated B cells express membrane-bound, soluble, and cytoplasmic isoforms of SLAM.
SLAM-SLAM interactions enhance B cell expansion and differentiation.
The extracellular domain of CD150 serves as a receptor for measles virus and acts as a co-activator for T and B cells.
Interactions occur with SH2D1A and PTPN11 via the cytoplasmic domain.
Mutations in the CD150 gene may be associated with X-linked lymphoproliferative disease (XLP).

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.