Thermo Fisher Scientific Alpha SNAP Polyclonal Antibody
Western blot, IHC, ELISA, IP 등 다양한 응용에 적합한 rabbit polyclonal antibody. Human, mouse 등 여러 종과 반응하며, 합성 펩타이드(aa 195–245) 면역원 사용. 액상 형태로 제공되며 -20°C에서 보관.
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Applications and Tested Dilutions
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:500–1:1,000 |
| Immunohistochemistry (IHC) | 1:50–1:250 |
| ELISA | 1:10,000 |
| Immunoprecipitation (IP) | 1:50–1:250 |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Human, Mouse, Non-human primate, Pig, Rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Synthetic peptide within amino acid region 195–245 on human alpha-soluble NSF attachment protein |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 0.5–1.5 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | Proprietary buffer, pH 7.4–7.8, with 0.5% BSA, 30% glycerol |
| Contains | 0.02% sodium azide |
| Storage Conditions | –20°C |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Target Information
SNAPs (soluble NSF attachment proteins) act together with SNAREs (SNAP receptors) and the N-ethylmaleimide-sensitive fusion protein (NSF) to mediate the fusion of transport vesicles with target membranes. These processes are essential for synaptic transmission, intra-Golgi transport, endosome-to-endosome fusion, and transcytotic vesicle-to-plasma membrane transport. Vesicle docking occurs when vesicle SNARE binds to its cognate target membrane SNARE, after which α-SNAP (or β-SNAP in brain) binds to the complex, recruits NSF, and forms a 20S fusion particle. ATP hydrolysis by NSF then initiates the membrane fusion process.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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