Thermo Fisher Scientific DDX1 Polyclonal Antibody
Rabbit 유래 다클론 항체로 DDX1 단백질 검출에 사용. Western blot, IHC, IP 등 다양한 응용에 적합. 인간 DDX1의 500–550번 잔기 영역을 면역원으로 사용. 액상 형태, 4°C 보관, 1년 권장 보관기간. 마우스, 랫, 소, 오랑우탄과 교차 반응 예측.
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Applications and Tested Dilution
| Application | Tested Dilution | Notes |
|---|---|---|
| Western Blot (WB) | 1:1,000–1:10,000 | |
| Immunohistochemistry (IHC) | 1:100–1:500 | |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:100–1:500 | |
| Immunoprecipitation (IP) | Assay-dependent |
Product Specifications
| Specification | Description |
|---|---|
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Region between residues 500 and 550 of human DEAD (Asp-Glu-Ala-Asp) Box Polypeptide 1 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 0.20 mg/mL |
| Storage Conditions | 4°C |
| Shipping Conditions | Wet ice |
Product Specific Information
- Recommended shelf life: 1 year from date of receipt
- For IHC, epitope retrieval with citrate buffer (pH 6.0) is recommended for FFPE tissue sections
- Based on 100% sequence identity, this antibody is predicted to react with Mouse, Rat, Bovine, and Orangutan
Target Information
DDX1 (ATP-dependent RNA helicase DDX1) acts as an ATP-dependent RNA helicase capable of unwinding both RNA-RNA and RNA-DNA duplexes.
It possesses 5′ single-stranded RNA overhang nuclease activity and ATPase activity on various RNA, but not DNA polynucleotides.
DDX1, together with RELA, acts as a coactivator to enhance NF-kappa-B-mediated transcriptional activation and serves as a positive regulator of cyclin CCND2 expression by binding to its promoter region.
It is a component of a multi-helicase-TICAM1 complex that functions as a cytoplasmic sensor of viral double-stranded RNA and plays a role in antiviral response activation, including induction of proinflammatory cytokines via TICAM1.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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