Thermo Fisher Scientific CD33 Monoclonal Antibody (OTI1E10), TrueMAB
인간 CD33 단백질을 인식하는 Mouse IgG1 단클론 항체로, WB, ICC/IF, Flow Cytometry에 사용 가능. Lyophilized 형태로 제공되며, 재구성 후 1 mg/mL 농도 유지. 급성 골수성 백혈병 연구 및 CD33 관련 세포 표지에 적합.
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Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:200–1:4000 |
| Immunocytochemistry (ICC/IF) | 1:100 |
| Flow Cytometry (Flow) | Assay-dependent |
Product Specifications
| Specification | Detail |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Clone | OTI1E10 |
| Immunogen | Full length human recombinant protein of human CD33 produced in HEK293T cells |
| Conjugate | Unconjugated |
| Form | Lyophilized |
| Concentration | 1 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.3, with 8% trehalose |
| Contains | No preservative |
| Storage Conditions | -20°C, Avoid Freeze/Thaw Cycles |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Additional Formats
Product Specific Information
For reconstitution, add 100 µL distilled water to achieve a final antibody concentration of approximately 1 mg/mL.
For conjugation experiments, perform an additional desalting step using Zeba Spin Desalting Columns (7K MWCO, 0.5 mL, Product #89882).
Target Information
CD33 is a transmembrane protein of the Siglec family, containing immunoreceptor tyrosine-based inhibitory motifs (ITIMs) that recruit SHP-1 and SHP-2 phosphatases. These motifs also mediate ubiquitin-dependent receptor removal from the cell surface.
CD33 is expressed on myelomonocytic cells, binds sialic acid residues in N- and O-glycans, and serves as a therapeutic target for acute myeloid leukemia. It is found on granulocyte and macrophage precursors in bone marrow but not on pluripotent stem cells. CD33 is also expressed on peripheral monocytes and is a useful marker for distinguishing myelogenous leukemia cells from lymphoid or erythroid leukemias.
Diseases associated with CD33 dysfunction include gallbladder lymphoma and extracutaneous mastocytoma.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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