Thermo Fisher Scientific AIRE Monoclonal Antibody (TM-724), eBioscience

Applications and Tested Dilution

Product Specifications

Additional Formats

• Biotin conjugate: Cat. No. 13-9534-82
• eFluor 570 conjugate: Cat. No. 41-9534-82
• Custom conjugation available upon request

Product Specific Information

This TM-724 monoclonal antibody reacts with human Autoimmune Regulator (AIRE), a 58 kDa transcription factor highly expressed in the perinuclear region of mature medullary thymic epithelial cells (mTECs). AIRE mRNA expression has also been observed in lymph nodes, fetal liver, intestines, dendritic cells, epithelial cells, and PBMCs.

AIRE is involved in thymocyte development and regulates tissue-specific antigen expression during negative selection. It also influences chemokine expression and antigen presentation. Three isoforms (AIRE-1, AIRE-2, AIRE-3) are known, and TM-724 is predicted to recognize all of them. Mutations in AIRE cause autoimmune-polyendocrinopathy-candidiasis-ectodermal-dystrophy (APECED) and autoimmune polyglandular syndrome type 1 (APS-1).

Applications Reported:
Suitable for immunohistochemical staining of frozen and formalin-fixed paraffin-embedded tissue sections, and for immunocytochemistry.

Applications Tested:
Validated in IHC on FFPE human thymus at ≤5 µg/mL. Low pH antigen retrieval is optimal, though high pH retrieval can also be used. Optimal dilution should be determined experimentally.

Purity: >90% (SDS-PAGE)
Aggregation: <10% (HPLC)
Filtration: 0.2 µm post-manufacturing filtered

Target Information

AIRE is a transcriptional regulator that binds DNA as a dimer or tetramer and regulates gene expression. The 545 amino acid nuclear protein contains an HSR domain, two PHD-type zinc fingers, and a SAND domain. It forms homodimers, homotetramers, and nuclear bodies, and interacts with transcriptional coactivators CBP and CREBBP. AIRE is involved in immune regulation and exhibits E3 ubiquitin ligase activity. Defects in AIRE cause the rare autosomal-recessive autoimmune disease APECED.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.