Thermo Fisher Scientific TIM17 Polyclonal Antibody

Applications

Tested Dilution

Publications

Western Blot (WB)

1:500-1:2,000

-

Immunoprecipitation (IP)

1-5 µL/mg of lysate

-

Product Specifications

Species Reactivity

Human

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

E. coli-derived Human TIMM17A fragment

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Purification

Antigen affinity chromatography, Protein A

Storage buffer

PBS, pH 7

Contains

0.03% ProClin 300

Storage conditions

-20° C, Avoid Freeze/Thaw Cycles

Shipping conditions

Wet ice

RRID

AB_2902904

Target Information

Translocation of nuclear encoded preproteins into the mitochondrial matrix requires the coordinated action of the translocases Tom and Tim, which are located in the outer mitochondrial membrane and the inner membrane, respectively. The mitochondrial preprotein translocases of the outer membrane (Tom) is a multi-subunit protein that contains at least eight proteins: four import receptor subunits (Tom70, Tom37, Tom22, and Tom20), three small proteins (Tom7, Tom6, and Tom5), and a structural component of the outer membrane channel (Tom40). The Tom machinery involves the import receptors, which initiate the binding of cytosolically synthesized preproteins to the outer membrane, and a general import pore (GIP), which promotes the translocation of various pre-proteins into the mitochondria. The TIM channel imports nuclear-encoded mitochondrial preproteins, and it involves three proteins, Tim17, Tim23 and Tim44, which are represented at equimolar ratios. Tim17 is expressed as two isoforms Tim17a and Tim17b, which differ only in their C termini sequences, and like Tim23, these proteins are ubiquitously expressed in fetal and adult tissues. Tim17 and Tim23 are integral membrane proteins that comprise the structural elements of the inner membrane channel by which the preproteins are transferred. The Tim44, on the other hand, is a largely hydrophilic protein that recruits the matrix located Hsp70 to the site where the preprotein emerges from the Tim channel.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.