Thermo Fisher Scientific RHEB Recombinant Rabbit Monoclonal Antibody (JG37-12)
RHEB 단백질을 인식하는 토끼 단클론 항체로, Western blot, IHC, ICC, Flow Cytometry에 사용 가능. 재조합 단백항체로 높은 특이성과 재현성을 제공. Protein A로 정제된 액상 형태이며, 인슐린/TOR 신호경로 연구에 적합.
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Applications and Tested Dilutions
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:500–1:2,000 |
| Immunohistochemistry (Paraffin) (IHC-P) | 1:50–1:200 |
| Immunocytochemistry / Immunofluorescence (ICC/IF) | 1:50–1:200 |
| Flow Cytometry (Flow) | 1:50–1:100 |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Rabbit / IgG |
| Expression System | HEK293 cells |
| Class | Recombinant Monoclonal |
| Type | Antibody |
| Clone | JG37-12 |
| Immunogen | Recombinant protein within Human RHEB (aa 21–108) |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Protein A |
| Storage Buffer | TBS, pH 7.4, with 40% glycerol, 0.05% BSA |
| Contains | 0.05% sodium azide |
| Storage Conditions | -20°C, avoid freeze/thaw cycles, store in dark |
| Shipping Conditions | Wet ice |
| RRID | AB_2848667 |
Product Specific Information
Positive Controls:
Mouse placenta tissue lysate, A431, SH-SY-5Y, human liver tissue, human colon tissue, human fetal skeletal muscle tissue.
Target Information
Gene / Protein: RHEB (Ras homolog enriched in brain)
Rheb encodes a member of the small GTPase superfamily and is a lipid-anchored, cell membrane protein containing five repeats of the RAS-related GTP-binding region. It plays a critical role in regulating growth and cell cycle progression via the insulin/TOR/S6K signaling pathway. The protein exhibits GTPase activity, cycling between GDP- and GTP-bound forms, and requires farnesylation for proper function. Three pseudogenes have been mapped: two on chromosome 10 and one on chromosome 22.
For Research Use Only.
Not for use in diagnostic procedures. Not for resale without express authorization.
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