Thermo Fisher Scientific Torc2 Monoclonal Antibody (5B10)
CRTC2 단백질을 타깃으로 하는 Mouse monoclonal antibody (Clone 5B10). Western blot, IHC, ICC, Flow cytometry, ELISA 등 다양한 응용에 적합. Human 및 비인간 영장류 시료에 반응. 연구용으로만 사용 가능.
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Applications and Tested Dilutions
| Application | Tested Dilution | Publications |
|---|---|---|
| Western Blot (WB) | 1:500–1:2,000 | - |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:200–1:1,000 | - |
| Immunocytochemistry (ICC/IF) | 1:200–1:1,000 | 1 publication |
| Flow Cytometry (Flow) | 1:200–1:400 | - |
| ELISA | 1:10,000 | - |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Human, Non-human primate |
| Published Species | Not Applicable |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Clone | 5B10 |
| Immunogen | Purified recombinant fragment of human CRTC2 expressed in E. coli |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | Not determined |
| Storage Buffer | Ascites |
| Contains | 0.03% sodium azide |
| Storage Conditions | Store at 4°C for short term. For long term storage, store at -20°C. Avoid freeze/thaw cycles. |
| Shipping Conditions | Wet ice |
| RRID | AB_10984517 |
Product Specific Information
MA5-15710 targets CRTC2 in indirect ELISA, FACS, IF, IHC, and WB applications, showing reactivity with Human and Non-human primate samples.
The immunogen is a purified recombinant fragment of human CRTC2 expressed in E. coli.
This antibody detects CRTC2 with a predicted molecular weight of approximately 80 kDa.
Target Information
CRTC2, also known as TORC2 (Transducers of Regulated cAMP Response Element-Binding (CREB) 2), along with CRTC1, acts as a potent CREB coactivator. It is exported from the nucleus in a CRM1-dependent manner through phosphorylation-dependent interactions.
CRTC2 phosphorylation and nuclear/cytoplasmic shuttling are key in regulating gluconeogenesis by cAMP.
CRTC2 is expressed in both B and T lymphocytes and abundantly in the thymus, playing a critical role in regulating genes involved in cellular energy metabolism.
CRTC1, a related protein, is essential for energy balance and fertility.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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