Thermo Fisher Scientific GPX4 Polyclonal Antibody
GPX4 단백질을 인식하는 Thermo Fisher Scientific의 염소 유래 폴리클로날 항체로, Western blot, IHC, ICC 등 다양한 응용에 사용 가능. 인간, 생쥐, 랫드에 반응하며, 고순도 정제 및 안정적 액상 형태로 제공. 세포 산화 스트레스 연구에 적합.
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Applications and Tested Dilution
| Application | Tested Dilution | Publications |
|---|---|---|
| Western Blot (WB) | 0.1–1 µg/mL | View 2 publications |
| Immunohistochemistry (IHC) | – | View 1 publication |
| Immunohistochemistry (Paraffin) (IHC (P)) | 4–6 µg/mL | – |
| Immunocytochemistry (ICC/IF) | – | View 1 publication |
Product Specifications
| Property | Description |
|---|---|
| Species Reactivity | Human, Mouse, Rat |
| Published Species | Bovine |
| Host / Isotype | Goat / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Synthetic peptide sequence (EEPLVIEKDLPHY) corresponding to the C-terminus amino acids of GPX4 (aa 184–196) |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 0.5 mg/mL |
| Purification | Ammonium sulfate precipitation |
| Storage Buffer | TBS, pH 7.3, with 0.5% BSA |
| Contains | 0.02% sodium azide |
| Storage Conditions | –20°C, Avoid Freeze/Thaw Cycles |
| Shipping Conditions | Wet ice |
| RRID | AB_10982478 |
Product Specific Information
This antibody is predicted to react with bovine, mouse, porcine, and rat based on sequence homology.
It is tested in Peptide ELISA with an antibody detection limit dilution of 1:16,000.
Target Information
Glutathione peroxidase (GPX4) catalyzes the reduction of hydrogen peroxide, organic hydroperoxide, and lipid peroxides by reduced glutathione, protecting cells against oxidative damage.
Human plasma glutathione peroxidase is a selenium-containing enzyme in which the UGA codon is translated into selenocysteine.
Through alternative splicing and transcription initiation, rat produces proteins that localize to the nucleus, mitochondrion, and cytoplasm.
In humans, experimental evidence for alternative splicing exists; alternative transcription initiation and the cleavage sites of the mitochondrial and nuclear transit peptides need to be experimentally verified.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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