Thermo Fisher Scientific GFAP Polyclonal Antibody
GFAP 단백질을 검출하기 위한 Thermo Fisher Scientific의 Rabbit Polyclonal Antibody. 인간, 마우스, 랫트 등 다양한 종에서 반응. Western blot, IHC, ICC/IF 등 여러 응용에 적합. 항원 친화 크로마토그래피로 정제된 고품질 항체.
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Applications and Tested Dilutions
| Application | Tested Dilution | Publications |
|---|---|---|
| Western Blot (WB) | 1:500–1:3,000 | – |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:100–1:1,000 | – |
| Immunohistochemistry (Frozen) (IHC (F)) | 1:100–1:1,000 | 1 publication |
| Immunocytochemistry (ICC/IF) | 1:100–1:1,000 | – |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Human, Mouse, Rat |
| Published Species | Mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Recombinant protein encompassing a sequence within the center region of human GFAP |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 0.72 mg/mL |
| Purification | Antigen affinity chromatography |
| Storage Buffer | PBS, pH 7, with 20% glycerol |
| Contains | 0.025% ProClin 300 |
| Storage Conditions | Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles. |
| Shipping Conditions | Wet ice |
| RRID | AB_2792257 |
Product Specific Information
- Keep as concentrated solution.
- Predicted reactivity: Mouse (94%), Rat (94%), Pig (95%), Bovine (97%).
- Positive Control: U87-MG, mouse brain, rat brain, GFAP-transfected 293T.
- Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.
Target Information
GFAP (Glial fibrillary acidic protein) is a member of the class III intermediate filament protein family. GFAP is heavily and specifically expressed in astrocytes and certain astroglia of the central nervous system, in satellite cells of peripheral ganglia, and in non-myelinating Schwann cells of peripheral nerves. Neural stem cells also strongly express GFAP. Antibodies to GFAP are useful markers of astrocytic cells and are expressed in many brain tumors derived from astrocytic cells. GFAP is also found in the lens epithelium, Kupffer cells of the liver, certain salivary tumor cells, and erythrocytes. Mutations in this gene cause Alexander disease, a rare disorder of astrocytes in the central nervous system. Alternative splicing of the GFAP gene results in multiple transcript variants encoding distinct isoforms.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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