Thermo Fisher Scientific Nanog Polyclonal Antibody
Nanog 단백질을 표적하는 Rabbit Polyclonal 항체로, 인간 및 생쥐 시료에 반응합니다. Western blot, IHC, ICC, ELISA, IP 등 다양한 응용에 적합하며 항원 친화 크로마토그래피로 정제되었습니다. 줄기세포 연구 및 분화 조절 연구에 활용 가능합니다.
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Applications and Tested Dilutions
| Application | Tested Dilution | Publications |
|---|---|---|
| Western Blot (WB) | 1:500–1:3,000 | View 1 publication |
| Immunohistochemistry (IHC) | – | View 1 publication |
| Immunohistochemistry (Paraffin) (IHC (P)) | Assay-dependent | – |
| Immunohistochemistry (Frozen) (IHC (F)) | Assay-dependent | – |
| Immunocytochemistry (ICC/IF) | 1:100–1:1,000 | View 1 publication |
| ELISA | 1:1,000–1:10,000 | – |
| Immunoprecipitation (IP) | 1:100–1:500 | – |
Product Specifications
| Item | Description |
|---|---|
| Species Reactivity | Human, Mouse |
| Published Species | Human, Mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Recombinant protein encompassing a sequence within the center region of human NANOG |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 0.5 mg/mL |
| Purification | Antigen affinity chromatography |
| Storage Buffer | PBS, pH 7, with 20% glycerol, 1% BSA |
| Contains | 0.01% thimerosal |
| Storage Conditions | Store at 4°C short term. For long term, store at -20°C, avoiding freeze/thaw cycles. |
| Shipping Conditions | Wet ice |
| RRID | AB_2792258 |
Product Specific Information
- Keep as concentrated solution.
- Predicted reactivity: Chimpanzee (98%).
- Positive Control: HeLa nucleus, NIH-3T3, mouse ESC.
- Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.
Target Information
NANOG (Nanog homeobox) is a divergent homeodomain protein that directs pluripotency and differentiation of undifferentiated embryonic stem cells.
NANOG mRNA is expressed in pluripotent mouse and human cell lines but absent in differentiated cells. Human NANOG shares 52% overall amino acid identity with the mouse protein and 85% within the homeodomain.
Human NANOG maps to gene locus 12p13.31, while mouse NANOG maps to 6 F2. Expression is detected in the inner cell mass of the blastocyst.
Research indicates that high levels of NANOG expression are found in undifferentiated N-Tera embryonal carcinoma cell lines. NANOG functions as a transcription regulator involved in proliferation and self-renewal of embryonic stem cells.
It plays a crucial role in stem cell maintenance and may be useful in generating induced pluripotent stem (iPS) cells for regenerative medicine. Overexpression of NANOG promotes S phase entry and proliferation.
Diseases associated with NANOG dysfunction include teratocarcinoma and germ cell/embryonal cancer.
⚠ WARNING: This product can expose you to chemicals including mercury, known to cause birth defects or other reproductive harm.
For more information, visit www.P65Warnings.ca.gov.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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