Thermo Fisher Scientific ZNF521 Polyclonal Antibody
ZNF521 단백질을 인식하는 Rabbit Polyclonal 항체로 Western blot, IHC, ICC/IF에 사용 가능. 인간 및 생쥐 시료에 반응하며 항원 친화 크로마토그래피로 정제됨. PBS 및 글리세롤 완충액에 보관되며 연구용으로 적합.
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Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | Assay-dependent |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:400 |
| Immunocytochemistry (ICC/IF) | 1:40 |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human, Mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | A synthetic peptide made to an internal portion of the human ZNF521 protein (between residues 450–550) [UniProt Q96K83] |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1.11 mg/mL |
| Purification | Antigen affinity chromatography |
| Storage Buffer | PBS with 30% glycerol |
| Contains | 0.05% sodium azide |
| Storage Conditions | Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles. |
| Shipping Conditions | Wet ice |
| RRID | AB_2718838 |
Target Information
The zinc finger protein 521 (ZNF521) is a transcription factor containing an N-terminal transcriptional repressor motif and 30 zinc finger domains. It plays a role in erythroid cell and osteoblast differentiation during development, inhibiting the activities of early B-cell factor 1 (EBF1) in erythroid cells and Runx2 in osteoblast precursors. ZFP521 binds to both Runx2 and histone deacetylase 3 (HDAC3), promotes their association, and antagonizes Runx2 transcriptional activity in a HDAC3-dependent manner, thereby regulating osteoblast differentiation, skeletal development, and bone homeostasis.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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