Thermo Fisher Scientific PRDX5 Recombinant Rabbit Monoclonal Antibody (125)

Applications

ELISA (ELISA)

• Tested Dilution: 1:5,000–1:10,000

Product Specifications

Product Specific Information

This product is preservative-free. It is recommended to add sodium azide to prevent contamination (final concentration 0.05%–0.1%).

Recombinant rabbit monoclonal antibodies are produced using in vitro expression systems. Antibody DNA sequences from immunoreactive rabbits are cloned and screened to select optimal candidates.
Advantages include:

• Superior specificity and sensitivity
• Lot-to-lot consistency
• Animal origin-free formulations
• Broader immunoreactivity due to the rabbit immune repertoire

This antibody specifically recognizes Human Peroxiredoxin 5 (PRDX5).

Target Information

Peroxiredoxin (Prx) is a family of peroxidases involved in cell proliferation, differentiation, and apoptosis.
Mammalian Prx proteins (approximately 50 isoforms) contain active site cysteine residues and exhibit thiol-specific antioxidant activity.
Mammalian Prxs are divided into:

• Typical 2-Cys Prx
• Atypical 2-Cys Prx
• 1-Cys Prx

Except for Prx VI (1-Cys subgroup), other 2-Cys Prx isoforms exhibit thioredoxin-dependent peroxidase (TPx) activity using thioredoxin, thioredoxin reductase, and NADPH as a reducing system.
Mammalian Prxs (20–30 kDa) vary in subcellular localization:

• Prx I, II, VI: cytosol
• Prx III: mitochondria
• Prx IV: ER and secretion
• Prx V: peroxisome, mitochondria, and cytosol

For Research Use Only.
Not for use in diagnostic procedures or resale without authorization.