Thermo Fisher Scientific NKCC1 (SLC12A2) (extracellular) Polyclonal Antibody
NKCC1 단백질을 인식하는 Thermo Fisher Scientific의 토끼 폴리클로날 항체로, Western blot, IHC, ICC, Flow cytometry 등 다양한 응용에 사용 가능. 인간, 마우스, 랫트 반응성. 항원 친화 크로마토그래피로 정제되었으며, 동결건조 형태로 제공.
- 카탈로그번호
- ANT-071-xxxxx (3개 옵션)
- 판매단위
- pk
카탈로그
3개 옵션 · 카탈로그 번호를 클릭하면 복사됩니다Thermo Fisher Scientific · Thermo Fisher Scientific NKCC1 (SLC12A2) (extracellular) Polyclonal Antibody
Applications and Tested Dilutions
| Application | Tested Dilution / Amount |
|---|---|
| Western Blot (WB) | 1:200 |
| Immunohistochemistry (Frozen) (IHC (F)) | 1:100 |
| Immunocytochemistry (ICC/IF) | 1:50 |
| Flow Cytometry (Flow) | 2.5 µg |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human, Mouse, Rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | (C)RRQAMKEMSIDQAK, corresponding to amino acid residues 828–841 of human NKCC1, extracellular loop |
| Conjugate | Unconjugated |
| Form | Lyophilized |
| Concentration | 0.6 mg/mL |
| Purification | Antigen affinity chromatography |
| Storage Buffer | PBS, pH 7.4, with 1% BSA |
| Contains | 0.05% sodium azide |
| Storage Conditions | -20°C, Avoid Freeze/Thaw Cycles |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Product Specific Information
Reconstitution: Add 25 µL, 50 µL, or 0.2 mL of double distilled water (DDW), depending on sample size.
The antibody is shipped as a lyophilized powder at room temperature. Upon arrival, store at -20°C.
Reconstituted solution can be stored at 4°C for up to 1 week. For long-term storage, aliquot and keep at -20°C.
Avoid multiple freeze/thaw cycles. Centrifuge all antibody preparations before use (10,000 × g, 5 min).
Target Information
NKCC1 mediates sodium and chloride transport and reabsorption. It is a membrane protein crucial for maintaining ionic balance and cell volume. NKCC1 functions as an electrically silent transporter and is phosphorylated in response to DNA damage.
Three transcript variants encoding two different isoforms have been identified for NKCC1.
Diseases associated with SLC12A2 include Basosquamous Carcinoma and Metatypical Basal Cell Carcinoma.
For Research Use Only.
Not for use in diagnostic procedures. Not for resale without express authorization.
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