Thermo Fisher Scientific CD47 Monoclonal Antibody (B6H12), eFluor 450, eBioscience
인간 CD47 단백질을 인식하는 B6H12 클론의 마우스 모노클로날 항체로, eFluor 450 형광체로 결합되어 Flow Cytometry에 최적화됨. 세포 부착 및 식세포 억제 신호 연구에 활용 가능하며, 405 nm 레이저에서 445 nm 방출 특성.
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Thermo Fisher Scientific CD47 Monoclonal Antibody (B6H12), eFluor 450, eBioscience
Applications and Tested Dilutions
| Application | Tested Dilution | Publications |
|---|---|---|
| Immunohistochemistry (IHC) | - | View 2 publications |
| Immunocytochemistry (ICC/IF) | - | View 2 publications |
| Flow Cytometry (Flow) | 5 µL (1.0 µg)/test | View 10 publications |
| Inhibition Assays (IA) | - | View 2 publications |
Product Specifications
| Item | Description |
|---|---|
| Species Reactivity | Human |
| Published Species | Not Applicable |
| Host / Isotype | Mouse / IgG1, kappa |
| Recommended Isotype Control | Mouse IgG1 kappa Isotype Control (P3.6.2.8.1), eFluor™ 450, eBioscience™ |
| Class | Monoclonal |
| Type | Antibody |
| Clone | B6H12 |
| Conjugate | eFluor™ 450 |
| Excitation / Emission Max | 405 / 445 nm |
| Form | Liquid |
| Concentration | 5 µL/test |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.2, with 0.2% BSA |
| Contains | 0.09% sodium azide |
| Storage Conditions | 4°C, store in dark, DO NOT FREEZE |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2848387 |
Available Formats
- Unconjugated
- Alexa Fluor 488
- Alexa Fluor 700
- APC
- APC-eFluor 780
- FITC
- Functional Grade
- NovaFluor Blue 660-120S
- NovaFluor Red 700 / 725 / 755
- NovaFluor Yellow 610 / 660
- PE-Cyanine5 / PE-Cyanine7
- PerCP-eFluor 710
- Request custom conjugation available
Product Specific Information
The monoclonal antibody B6H12 reacts to CD47, also known as integrin-associated protein (IAP) and neurophilin.
CD47 is a glycosylated five-transmembrane protein involved in adhesion through interactions with SIRP (signal regulator protein) and integrins CD51/CD61 and CD41/CD61.
It mediates bi-directional signaling to regulate synaptic activity and macrophage phagocytosis. CD47 also serves as a receptor for thrombospondin, influencing T cell activation or apoptosis.
This antibody exhibits neutralizing activity and is validated for Flow Cytometric analysis.
Pre-diluted and tested on normal human peripheral blood cells at 5 µL (1.0 µg) per test.
A test is defined as the amount that stains a cell sample in 100 µL final volume (10⁵–10⁸ cells/test).
eFluor 450 is an alternative to Pacific Blue, excited at 405 nm and emitting at 445 nm (violet laser compatible).
Target Information
CD47 (integrin-associated protein, IAP) is a ubiquitously expressed transmembrane glycoprotein interacting with integrins and modulating signaling pathways.
It regulates intracellular calcium levels upon cell adhesion to extracellular matrix and serves as a receptor for thrombospondin.
CD47 expression is broad across tissues, reduced on Rh erythrocytes, and acts as a ligand for CD172a (SIRPα), preventing phagocytosis of CD47-positive cells.
It influences cell migration, B cell adhesion, T cell activation, chondrocyte mechanotransduction, and neuronal development, particularly in synapse-rich regions of the brain and retina.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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