Thermo Fisher Scientific CD47 Monoclonal Antibody (B6H12), eFluor 450, eBioscience

Thermo Fisher Scientific CD47 Monoclonal Antibody (B6H12), eFluor 450, eBioscience

Applications and Tested Dilutions

Product Specifications

Available Formats

• Unconjugated
• Alexa Fluor 488
• Alexa Fluor 700
• APC
• APC-eFluor 780
• FITC
• Functional Grade
• NovaFluor Blue 660-120S
• NovaFluor Red 700 / 725 / 755
• NovaFluor Yellow 610 / 660
• PE-Cyanine5 / PE-Cyanine7
• PerCP-eFluor 710
• Request custom conjugation available

Product Specific Information

The monoclonal antibody B6H12 reacts to CD47, also known as integrin-associated protein (IAP) and neurophilin.
CD47 is a glycosylated five-transmembrane protein involved in adhesion through interactions with SIRP (signal regulator protein) and integrins CD51/CD61 and CD41/CD61.
It mediates bi-directional signaling to regulate synaptic activity and macrophage phagocytosis. CD47 also serves as a receptor for thrombospondin, influencing T cell activation or apoptosis.

This antibody exhibits neutralizing activity and is validated for Flow Cytometric analysis.
Pre-diluted and tested on normal human peripheral blood cells at 5 µL (1.0 µg) per test.
A test is defined as the amount that stains a cell sample in 100 µL final volume (10⁵–10⁸ cells/test).

eFluor 450 is an alternative to Pacific Blue, excited at 405 nm and emitting at 445 nm (violet laser compatible).

Target Information

CD47 (integrin-associated protein, IAP) is a ubiquitously expressed transmembrane glycoprotein interacting with integrins and modulating signaling pathways.
It regulates intracellular calcium levels upon cell adhesion to extracellular matrix and serves as a receptor for thrombospondin.
CD47 expression is broad across tissues, reduced on Rh erythrocytes, and acts as a ligand for CD172a (SIRPα), preventing phagocytosis of CD47-positive cells.
It influences cell migration, B cell adhesion, T cell activation, chondrocyte mechanotransduction, and neuronal development, particularly in synapse-rich regions of the brain and retina.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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