Thermo Fisher Scientific CD45RA Monoclonal Antibody (HI100), eFluor 450, eBioscience
인간 CD45RA 항원을 인식하는 HI100 클론 단일클론 항체로, eFluor 450 형광체가 결합된 제품입니다. 유세포분석(Flow Cytometry)에 최적화되어 있으며, 405 nm 레이저로 여기되어 445 nm에서 방출됩니다. 인간 면역세포 아형 분석에 적합합니다.
- 카탈로그번호
- 48-0458-4x (2개 옵션)
- 판매단위
- pk
카탈로그
2개 옵션 · 카탈로그 번호를 클릭하면 복사됩니다Thermo Fisher Scientific · Thermo Fisher Scientific CD45RA Monoclonal Antibody (HI100), eFluor 450, eBioscience
Thermo Fisher Scientific CD45RA Monoclonal Antibody (HI100), eFluor 450, eBioscience
Applications and Tested Dilution
- Application: Flow Cytometry (Flow)
- Tested Dilution: 5 µL (0.5 µg)/test
- Publications: View 23 publications
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human |
| Published Species | Human |
| Host / Isotype | Mouse / IgG2b, kappa |
| Recommended Isotype Control | Mouse IgG2b kappa Isotype Control (eBMG2b), eFluor™ 450 |
| Class | Monoclonal |
| Type | Antibody |
| Clone | HI100 |
| Conjugate | eFluor™ 450 |
| Excitation / Emission Max | 405 / 445 nm |
| Form | Liquid |
| Concentration | 5 µL/Test |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.2, with 0.2% BSA |
| Contains | 0.09% sodium azide |
| Storage Conditions | 4°C, store in dark, DO NOT FREEZE |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_1272129 |
Available Formats
- Unconjugated
- Alexa Fluor 532
- Alexa Fluor 700
- Alexa Fluor Plus 555
- Alexa Fluor Plus 750
- APC
- APC-eFluor 780
- Biotin
- Brilliant UV 395, 496, 563, 661, 737
- Brilliant Violet 421, 786
- eFluor 506
- FITC
- NovaFluor Blue 660-120S, Blue 800, Red 710, UV 765, Violet 690, Violet 800, Yellow 610, Yellow 660, Yellow 810
- PE, PE-Cyanine5, PE-Cyanine7, PE-eFluor 610
- PerCP-Cyanine5.5
- Super Bright 436, 600, 645, 702
- Request custom conjugation
Product Specific Information
The HI100 monoclonal antibody reacts with human CD45RA, a 220 kDa molecule expressed by subpopulations of CD4+ and CD8+ peripheral T lymphocytes, and B cells. CD45RA+ T cell populations are mainly naive/virgin, allowing use as a phenotypic marker to discriminate T cell subsets.
- Applications Reported: Flow cytometric analysis
- Applications Tested: Pre-titrated and tested with normal human peripheral mononuclear cells at 5 µL (0.5 µg) per test
- Test Definition: Amount of antibody that stains a cell sample in 100 µL final volume (10⁵–10⁸ cells/test)
- Fluorophore: eFluor® 450 (alternative to Pacific Blue®)
- Excitation: 405 nm
- Emission: 445 nm
- Laser: Violet
- Filtration: 0.2 µm post-manufacturing filtered
Target Information
CD45RA is an isoform of the CD45 complex with restricted expression among lymphoid subtypes. CD45 (LCA, leukocyte common antigen) is a receptor-type protein tyrosine phosphatase expressed in all nucleated hematopoietic cells, representing ~10% of lymphocyte surface proteins. It regulates Src-family kinases and plays a key role in lymphocyte development, antigen signaling, and integrin-mediated pathways. CD45 isoforms differ by extracellular domain and vary by cell type and physiological state.
CD45 dysfunction is associated with severe combined immunodeficiency and Hepatitis C.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
제품 이미지
Thermo Fisher Scientific 상품 둘러보기
전체보기
문의
0개 · 배송·재고 문의는 실시간 상담이 빠릅니다아직 등록된 문의가 없어요.