Thermo Fisher Scientific Cytokeratin 10 Recombinant Mouse Monoclonal Antibody (rKRT10, 1275)
Cytokeratin 10을 인식하는 재조합 마우스 단클론 항체로, IHC(파라핀 절편)에 적합합니다. 인간, 개, 고양이에 반응하며 HEK293 세포에서 발현되었습니다. 고순도 Protein A/G 정제, 1 mg/mL 농도, 보존제 무첨가 PBS 용액 형태입니다.
- 판매단위
- pk
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Thermo Fisher Scientific Cytokeratin 10 Recombinant Mouse Monoclonal Antibody (rKRT10, 1275)
Applications
- Immunohistochemistry (Paraffin) (IHC (P))
Tested Dilution: 0.1–0.2 µg/mL
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Dog, Cat, Human |
| Host / Isotype | Mouse / IgG1, kappa |
| Expression System | HEK293 cells |
| Class | Recombinant Monoclonal |
| Type | Antibody |
| Clone | rKRT10, 1275 |
| Immunogen | Recombinant full-length human KRT10 protein |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Protein A/G |
| Storage Buffer | PBS, pH 7.4 |
| Contains | No preservative |
| Storage Conditions | -20°C or -80°C if preferred |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Product Specific Information
Immunohistochemistry (PFA fixed):
- Incubate antibody for 30 min at room temperature (RT).
- For formalin-fixed tissues, heat tissue sections in 10 mM Tris with 1 mM EDTA (pH 9.0) for 45 min at 95°C, followed by cooling at RT for 20 min.
Target Information
Cytokeratins (CK) are intermediate filaments of epithelial cells found in both keratinizing (e.g., skin) and non-keratinizing tissues (e.g., mesothelial cells). Although not typical endothelial markers, cytokeratins are present in some microvascular endothelial cells.
There are at least 20 cytokeratin types (40–70 kDa, pI 5–8.5), identified by 2D gel electrophoresis.
Cytokeratins are classified into two groups:
- Type I (acidic polypeptides)
- Type II (basic polypeptides)
Every epithelial cell expresses at least one member from each class.
Monoclonal antibodies to cytokeratin proteins are valuable tools for tumor identification and classification.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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