Thermo Fisher Scientific IL-3 Polyclonal Antibody
Human IL-3 단백질을 인식하는 Rabbit Polyclonal Antibody로, WB, IHC(P), ELISA에 사용 가능. 항원 친화 크로마토그래피로 정제되어 높은 특이성과 재현성을 제공. 동결건조 형태로 제공되며 재구성 후 500 µg/mL 농도로 사용. 연구용으로만 사용 가능.
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Applications and Tested Dilutions
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 0.1–0.5 µg/mL |
| Immunohistochemistry (Paraffin) (IHC (P)) | 0.5–1 µg/mL |
| ELISA | 0.1–0.5 µg/mL |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | E. coli-derived human IL-3 recombinant protein (Position: A20–F152) |
| Conjugate | Unconjugated |
| Form | Lyophilized |
| Concentration | 500 µg/mL |
| Purification | Antigen affinity chromatography |
| Storage Buffer | PBS |
| Contains | 0.05 mg sodium azide |
| Storage Conditions | -20°C |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2746613 |
Product Specific Information
Reconstitute with 0.2 mL of distilled water to yield a concentration of 500 µg/mL.
Target Information
Interleukin 3 (IL-3), also known as Mast Cell Growth Factor (MCGF), Multi-Colony Stimulating Factor (multi-CSF), or Eosinophil-CSF (E-CSF), is produced by activated T cells, mast cells, and eosinophils.
IL-3 is a hematopoietic growth factor that stimulates colony formation of erythroid, megakaryocyte, neutrophil, eosinophil, basophil, mast cell, and monocytic lineages.
Most of these functions are enhanced or dependent on co-stimulation with other cytokines.
Human and mouse IL-3 share only 29% sequence identity at the amino acid level, and the bioactivity of IL-3 is highly species specific (e.g., human IL-3 has no activity on mouse cells).
IL-3 supports proliferation of various hematopoietic cell types and is involved in cell growth, differentiation, and apoptosis. It has also been shown to possess neurotrophic activity and may be associated with neurologic disorders.
For Research Use Only.
Not for use in diagnostic procedures. Not for resale without express authorization.
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