Thermo Fisher Scientific DIAPH1 Polyclonal Antibody
Rabbit polyclonal antibody against DIAPH1 protein for detection in human samples. Validated for WB, IHC-P, and ICC/IF applications. High specificity with predicted cross-reactivity in mouse, rat, and pig. Supplied as liquid, antigen affinity purified, ...
- 카탈로그번호
- PA521409
- 판매단위
- pk
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Applications and Tested Dilutions
| Application | Tested Dilution | Publications |
|---|---|---|
| Western Blot (WB) | 1:500–1:3,000 | View 2 publications |
| Immunohistochemistry (Paraffin) (IHC-P) | 1:100–1:1,000 | – |
| Immunocytochemistry (ICC/IF) | 1:100–1:1,000 | View 1 publication |
Product Specifications
| Property | Description |
|---|---|
| Species Reactivity | Human |
| Published Species | Human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Recombinant fragment corresponding to amino acids 1–237 of DIAPH1 (Uniprot ID: O60610) |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 0.69 mg/mL |
| Purification | Antigen affinity chromatography |
| Storage Buffer | 0.1M Tris glycine, pH 7, with 10% glycerol |
| Contains | 0.01% thimerosal |
| Storage Conditions | Store at 4°C short term. For long-term storage, store at -20°C, avoiding freeze/thaw cycles. |
| Shipping Conditions | Wet ice |
| RRID | AB_11153295 |
Product Specific Information
- Recommended positive controls: A431, H1299, HeLa, HepG2, Molt-4, Raji
- Predicted reactivity: Mouse (95%), Rat (94%), Pig (98%)
- Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.
Target Information
The DIAPH1 gene is a homolog of the Drosophila diaphanous gene and has been associated with autosomal dominant, fully penetrant, nonsyndromic sensorineural progressive low-frequency hearing loss.
Actin polymerization involves proteins interacting with diaphanous protein in Drosophila and mouse, suggesting a possible regulatory role in actin polymerization in inner ear hair cells.
Alternatively spliced transcript variants encoding distinct isoforms have been identified for this gene.
⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause birth defects or other reproductive harm.
For more information, visit www.P65Warnings.ca.gov.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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