Thermo Fisher Scientific PDI Polyclonal Antibody

Applications

Tested Dilution

Publications

Western Blot (WB)

1:1,000

-

Immunohistochemistry (PFA fixed) (IHC (PFA))

1:1,000

-

Immunocytochemistry (ICC/IF)

1:100

View 1 publication 1 publication

Immunoprecipitation (IP)

Assay-Dependent

-

Product Specifications

Species Reactivity

Bovine, Dog, Guinea pig, Hamster, Human, Mollusc, Mouse, Sheep, Pig, Rat, Xenopus

Published species

Human

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Rat PDI synthetic peptide (409-509 aa C-terminal) conjugated to KLH if (typeof window.$mangular === undefined || !window.$mangular) { window.$mangular = {}; } $mangular.antigenJson = \[{targetFamily:PDI,uniProtId:P04785-1,ncbiNodeId:10114,antigenRange:409-509,antigenLength:509,antigenImageFileName:PA5-77821_PDI_P04785-1_Rabbit.svg,antigenImageFileNamePDP:PA5-77821_PDI_P04785-1_Rabbit_PDP.jpeg,sortOrder:2}\]; $mangular.isB2BCMGT = false; $mangular.isEpitopesModalImageMultiSizeEnabled = true;

View immunogen .st0{fill:#FFFFFF;} .st1{fill:#1E8AE7;}

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

1 mg/mL

Purification

Antigen affinity chromatography, Protein A

Storage buffer

PBS, pH 7.4, with 50% glycerol

Contains

0.09% sodium azide

Storage conditions

-20°C

Shipping conditions

Wet ice

RRID

AB_2736334

Product Specific Information

A 1:1,000 dilution of PA5-77821 was sufficient for detection of PDI in 20 µg of HeLa cell lysate by ECL immunoblot analysis.|Detects approximately 58kDa.

Target Information

Protein disulfide isomerase (PDI) is a multifunctional protein that catalyzes the formation, breakage and rearrangement of disulfide bonds. At the cell surface, the protein seems to act as a reductase that cleaves disulfide bonds of proteins attached to the cell. It may therefore cause structural modifications of exofacial proteins. Inside the cell, PDI seems to form/rearrange disulfide bonds of nascent proteins. At high concentrations, it functions as a chaperone that inhibits aggregation of misfolded proteins. At low concentrations, it facilitates aggregation (anti-chaperone activity). PDI may be involved with other chaperones in the structural modification of the TG precursor in hormone biogenesis. It also acts a structural subunit of various enzymes such as prolyl 4-hydroxylase and microsomal triacylglycerol transfer protein MTTP. Receptor for LGALS9; the interaction retains P4HB at the cell surface of Th2 T helper cells, increasing disulfide reductase activity at the plasma membrane, altering the plasma membrane redox state and enhancing cell migration.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.