Thermo Fisher Scientific HDAC6 Monoclonal Antibody (OTI5E6), TrueMAB
HDAC6 단백질을 인식하는 Mouse IgG1 단클론 항체로, Western blot, IHC, ICC, Flow cytometry 등에 사용 가능. 인간 시료에 반응하며, 동결건조 형태로 제공. 트레할로스 포함 PBS 버퍼에 보관하며, 연구용으로만 사용.
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Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:500–1:2,000 |
| Immunohistochemistry (Paraffin) (IHC-P) | 1:150 |
| Immunocytochemistry (ICC/IF) | 1:100 |
| Flow Cytometry (Flow) | 1:100 |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Clone | OTI5E6 |
| Immunogen | Full-length human recombinant HDAC6 protein produced in HEK293T cells |
| Conjugate | Unconjugated |
| Form | Lyophilized |
| Concentration | 1 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.3, with 8% trehalose |
| Contains | No preservative |
| Storage Conditions | -20°C, Avoid freeze/thaw cycles |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Product Specific Information
For reconstitution, add 100 µL of distilled water to obtain a final antibody concentration of approximately 1 mg/mL.
For conjugation experiments, perform an additional desalting step using Zeba Spin Desalting Columns (7K MWCO, 0.5 mL, Product #89882).
Target Information
HDAC6 is responsible for deacetylating lysine residues on the N-terminal part of core histones (H2A, H2B, H3, and H4).
Histone deacetylation acts as an epigenetic repression marker and plays a crucial role in transcriptional regulation, cell cycle progression, and development.
HDAC6 functions through large multiprotein complexes and is central in microtubule-dependent cell motility via tubulin deacetylation.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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