Thermo Fisher Scientific GATA3 Monoclonal Antibody (OTI1A5), TrueMAB
GATA3 단백질을 인식하는 Mouse 단일클론 항체로 Western blot 및 유세포분석에 사용 가능. Human, Mouse, Rat 반응성. 동결건조 형태로 제공되며, 재구성 후 1 mg/mL 농도. PBS 완충액(8% trehalose) 보존, -20°C 보관.
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Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:2,000 |
| Flow Cytometry (Flow) | Assay-dependent |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human, Mouse, Rat |
| Host / Isotype | Mouse / IgG2b |
| Class | Monoclonal |
| Type | Antibody |
| Clone | OTI1A5 |
| Immunogen | Human recombinant protein fragment corresponding to amino acids 155–443 of human GATA3 produced in E. coli |
| Conjugate | Unconjugated |
| Form | Lyophilized |
| Concentration | 1 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.3, with 8% trehalose |
| Contains | No preservative |
| Storage Conditions | -20°C, Avoid Freeze/Thaw Cycles |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Product Specific Information
For reconstitution, add 100 µL distilled water to achieve a final antibody concentration of approximately 1 mg/mL.
For conjugation experiments, perform an additional desalting step using Zeba Spin Desalting Columns (7K MWCO, 0.5 mL, Product #89882).
Target Information
GATA3 is a transcription factor that binds enhancer elements of T-cell receptor (TCR) genes (alpha, beta, gamma, delta). It plays a critical role in T-cell lineage differentiation and activation.
Originally identified as a zinc finger-containing enhancer-binding protein, GATA3 is expressed in T lymphocytes but not in B cells or macrophages.
The human GATA3 gene consists of six exons spanning approximately 17 kb, encoding two zinc finger domains highly conserved with GATA1.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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