Thermo Fisher Scientific NT5E Monoclonal Antibody (OTI1G2), TrueMAB
NT5E(CD73) 단백질을 인식하는 Thermo Fisher Scientific의 단클론 항체로, Western blot 및 IHC(P) 실험에 적합. 휴먼, 마우스, 랫트 반응성. 동결건조 형태로 제공되며, PBS 버퍼에 보관. 연구용으로만 사용 가능.
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Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:2,000 |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:150 |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human, Mouse, Rat |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Clone | OTI1G2 |
| Immunogen | Human recombinant protein fragment corresponding to amino acids 119–232 of human NT5E produced in E.coli. |
| Conjugate | Unconjugated |
| Form | Lyophilized |
| Concentration | 1 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.3, with 8% trehalose |
| Contains | No preservative |
| Storage Conditions | -20°C, Avoid Freeze/Thaw Cycles |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Product Specific Information
For reconstitution, add 100 µL of distilled water to achieve a final antibody concentration of approximately 1 mg/mL.
For conjugation experiments, perform an additional desalting step (e.g., Zeba Spin Desalting Columns, 7K MWCO, 0.5 mL, Product #89882).
Target Information
CD73 (Ecto-5'-nucleotidase, 5'-ribonucleotide phosphohydrolase) catalyzes the conversion of purine 5'-mononucleotides to nucleosides at neutral pH, with AMP as the preferred substrate.
It forms a dimer of two identical 70 kDa subunits attached to the plasma membrane via a glycosyl phosphatidylinositol linkage and serves as a marker of lymphocyte differentiation.
Deficiency in CD73 is associated with various immunodeficiency diseases.
The CD73 gene is localized to chromosome 6q14–q21. Mutations can cause calcification of joints and arteries, and intestinal tuberculosis.
Two transcript variants encoding different isoforms have been identified.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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