Thermo Fisher Scientific Titin Monoclonal Antibody (T11)

Applications

Tested Dilution

Publications

Western Blot (WB)

Assay-dependent

-

Immunohistochemistry (Frozen) (IHC (F))

1:1,000

-

Immunomicroscopy (IM)

Assay-dependent

-

Product Specifications

Species Reactivity

Chicken

Host/Isotype

Mouse / IgG2b

Class

Monoclonal

Type

Antibody

Clone

T11

Immunogen

Purified, full length, native, chicken titin protein from chicken breast muscle.

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

Conc. Not Determined

Storage buffer

Ascites

Contains

15mM sodium azide

Storage conditions

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Shipping conditions

Wet ice

RRID

AB_2211848

Product Specific Information

Recommended positive controls: skeletal muscle.

Target Information

Monoclonal Anti-Titin can be used for study of the elastic filaments within sarcomeric structures. It is also useful as a differentiation marker in the separation of rhabdomyosarcomas from other muscle tumors. By indirect immunofluorescence the antibody displays a typical striated staining pattern on frozen sections of chicken skeletal and cardiac muscle tissues. Stains the region of the A-I junction by indirect immunofluorescence. It shows a decoration line 0.05 mm from the end of the A band in electron micro-graphs. In immunoblotting using total extracts of chicken breast muscle the antibody reacts specifically with both bands of the titin doublet, and showed no reaction with nebulin.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.