Gibco™

AAV-MAX Helper-Free AAV Production System Kit, without lysis buffer

The Gibco AAV-MAX Helper-Free AAV Production System is a scalable, high-titer platform for suspension AAV production. It is based on자세히 알아보기

The Gibco AAV-MAX Helper-Free AAV Production System is a scalable, high-titer platform for suspension AAV production. It is based on a high-density suspension culture of clonal 293F-derived Viral Production Cells 2.0 in Viral Production Medium. Optimal viral titers are mediated by our advanced lipid nanoparticle transfection reagent in combination with a novel production enhancer. The components are designed to work synergistically, resulting in maximal titers and eliminating the need to optimize reagents and protocols.

Features of the AAV-MAX Helper-Free AAV Production System include:
• Components work synergistically to produce high AAV titers greater than 5 x 1010 vg/mL (unpurified)
• Suspension-based system has scalable protocols from shake flask to bioreactor scale
• Simplified workflow—streamlined protocol using helper virus-free triple transfection
• Clonal, 293F-derived, high-producer cell line
• Robust transfection protocols are adaptable to large-scale transfection

The components included in the AAV-MAX System Kit are:
• 1 vial of Viral Production Cells 2.0
• 1 L of Viral Production Medium
• 1 AAV-MAX Transfection Kit for 1 L of production
• 100 mL Viral-Plex Complexation Buffer
• 100 mL GlutaMAX Supplement

All components are also available for purchase separately. Note, this version of the kit does not contain the AAV-MAX Lysis Buffer. To purchase the kit including the AAV-MAX Lysis Buffer, please see Cat. No. A51217.

사양

세포주Viral Production Cells 2.0

세포 유형Kidney (Embryonic)

Culture TypeSuspension Cell Culture

제품 유형AAV Production System

유통 기한12 months from date of manufacture

멸균Sterile-filtered

용도(애플리케이션)AAV production

제품라인AAV-MAX

수량1 Kit

Unit SizeEach