
Thermo Fisher Scientific Goat anti-Human IgM (Heavy chain) Recombinant Secondary Antibody, HRP
Goat anti-Human IgM (Heavy chain) HRP 결합 리콤비넌트 2차 항체로, 높은 특이성과 재현성을 제공합니다. 인간 IgM에만 반응하며 IgG, IgA 등과 교차 반응하지 않습니다. WB 및 ELISA에 적합하며, 단백질 G 정제 및 PBS 보존 용액으로 제공됩니다.
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Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:5000 |
| ELISA | 1:2000–1:4000 |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Goat / IgG |
| Expression System | Expi293 |
| Class | Recombinant Monoclonal |
| Type | Secondary Antibody |
| Clone | 22H20L13 |
| Immunogen | Purified human IgM |
| Conjugate | HRP |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Protein G |
| Storage Buffer | PBS, pH 7.4, with proprietary stabilizer |
| Contains | Proprietary preservative |
| Storage Conditions | 4°C, do not freeze |
| Shipping Conditions | Wet ice |
| RRID | AB_2910650 |
| Target | IgM (Heavy chain) |
| Antibody Form | Whole Antibody |
Additional Formats
- AP
- Biotin
- FITC
- TRITC
- Custom conjugation available upon request
Product Specific Information
This Goat anti-Human IgM (Heavy chain) Recombinant Monoclonal Secondary Antibody reacts specifically with human IgM heavy chain and shows no cross-reactivity with human IgG, IgA, or other species immunoglobulins, including monkey IgM.
Each lot’s sensitivity and specificity are validated using ELISA.
Recombinant antibodies are produced using cloned genes coding for the desired antibody, expressed in vitro.
Advantages include:
- Enhanced specificity
- Animal origin-free formulation
- Lot-to-lot consistency
Optimal performance requires careful titration in the assay of interest.
Target Information
Anti-Human secondary antibodies are affinity-purified and exhibit well-characterized specificity for human immunoglobulins.
They are useful for detection, sorting, and purification of specific targets.
Secondary antibodies enhance versatility and sensitivity by supporting multiple detection systems (e.g., HRP, AP, fluorescence) and enabling signal amplification.
Typically, secondary antibodies are generated by immunizing the host animal with pooled immunoglobulins from the target species, followed by purification and modification (e.g., immunoaffinity chromatography, fragmentation, label conjugation) to yield highly specific reagents.
For Research Use Only.
Not for use in diagnostic procedures. Not for resale without express authorization.
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