
Thermo Fisher Scientific Goat anti-Human IgM (Heavy chain) Recombinant Secondary Antibody
Thermo Fisher의 염소 유래 재조합 단클론 항-Human IgM 항체로, 인간 IgM heavy chain에 특이적입니다. Western blot과 ELISA에 적합하며, 높은 특이성과 재현성을 제공합니다. 동물 유래 성분이 없고 로트 간 일관성이 우수합니다.
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Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:5000 |
| ELISA | 1:1000 to 1:2000 |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Goat / IgG |
| Expression System | Expi293 |
| Class | Recombinant Monoclonal |
| Type | Secondary Antibody |
| Clone | 22H20L13 |
| Immunogen | Purified human IgM |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Protein G |
| Storage Buffer | PBS, pH 7.4 |
| Contains | 0.09% sodium azide |
| Storage Conditions | Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles. |
| Shipping Conditions | Wet ice |
| RRID | AB_2910648 |
| Target | IgM (Heavy chain) |
| Antibody Form | Whole Antibody |
Product Specific Information
This Goat anti-Human IgM (Heavy chain), Recombinant Monoclonal Antibody reacts specifically with the human IgM heavy chain, showing no cross-reactivity with human IgG, IgA, or immunoglobulins from other species including monkey IgM.
Each lot’s sensitivity and specificity are validated by ELISA.
Recombinant antibodies are generated using cloned genes encoding the desired antibody, expressed in vitro via an appropriate vector system. Advantages include improved specificity, animal-origin-free formulation, and consistent performance across lots.
It is recommended to titrate the antibody for optimal assay performance.
Target Information
Anti-Human secondary antibodies are affinity-purified reagents designed for specific detection of human immunoglobulins. They are useful for detection, sorting, or purification of target molecules and compatible with multiple detection systems (e.g., HRP, AP, fluorescence).
These antibodies provide enhanced sensitivity through signal amplification, as multiple secondary antibodies can bind to a single primary antibody.
Typically, secondary antibodies are generated by immunizing the host animal with pooled immunoglobulins from the target species and may be further purified or modified (e.g., immunoaffinity purification, fragmentation, or conjugation) to improve specificity and performance.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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