Thermo Fisher Scientific ErbB2 (HER-2) Monoclonal Antibody (2G11), Biotin, eBioscience

Applications and Tested Dilutions

Product Specifications

Available Formats

• Unconjugated
• FITC
• Request custom conjugation

Product Specific Information

The antibody (clone 2G11) can be used to stain cryostat sections or cell smears.
BMS120 is suitable as a primary antibody for FACS analysis.
BMS120FI can be used for direct staining of cells, while BMS120BT can be applied in a two-step procedure using the biotin-(strept)avidin system.

The epitope is located in the extracellular domain, and no cross-reactivity with EGF-R has been detected by immunoprecipitation.

HER-2 oncogene amplification and oncoprotein overexpression have been correlated with breast, ovarian, gastric, lung, and other cancers.
In 15–40% of primary breast cancers, HER-2 amplification is found and is highly correlated with overexpression of the encoded protein, playing a major role in the initiation of ductal carcinomas. HER-2 overexpression serves as an independent prognostic factor with greater predictive power than most current prognostic tools, especially in axillary lymph-node-positive breast cancer patients.

Approximately 32% of ovarian carcinomas overexpress the HER-2 oncoprotein, and survival of these patients is significantly worse compared with cases of normal HER-2 expression. Patients with high HER-2 expression also show lower complete response rates to primary therapy.
A correlation between HER-2 expression and clinical outcome has been demonstrated for head and neck, salivary gland, and placental carcinomas.

HER-2 is useful in identifying cancer cells with increased aggressiveness.
Soluble p97-115 HER-2 (the circulating fragment of p185 HER-2) levels in serum can be used as a diagnostic tool for monitoring tumor spread, postoperative relapse, and metastatic risk in various cancers.

Applications Tested: Flow Cytometry.

Target Information

ErbB2 (HER2) is a receptor tyrosine kinase overexpressed in certain breast tumors.
Herceptin, a monoclonal antibody targeting this kinase, is used in the treatment of metastatic HER2-positive cancers.

For Research Use Only. Not for use in diagnostic procedures.
Not for resale without express authorization.